D. H. Busch

A mass cytometry approach to track the evolution of T cell responses during infection and immunotherapy by paired T cell receptor repertoire and T cell differentiation state analysis

The paper develops a mass cytometry method to track T cell receptor repertoires and differentiation states during infection and immunotherapy, showing how antigen recognition influences T cell responses.

Dynamic monitoring of viral gene expression reveals rapid antiviral effects of CD8 T cells recognizing the HCMV-pp65 antigen

This study develops ARMATA, a method to measure antiviral T-cell activity against HCMV, revealing pp65 antigen recognition occurs before the late virus cycle phase.

Miniaturized CAR knocked onto CD3epsilon extends TCR function with CAR specificity under control of endogenous TCR signaling cascade

This paper describes a method to miniaturize CAR T cells by integrating only the antigen-binding domain under the control of the endogenous TCR signaling cascade, preserving TCR function while enabling CAR specificity.

TCR/CD3-based synthetic antigen receptors (TCC) convey superior antigen sensitivity combined with high fidelity of activation

This study shows TCR/CD3-based synthetic antigen receptors (TCC) have superior antigen sensitivity and activation fidelity compared to second-generation CARs.

Activation-inducible CAR expression enables precise control over engineered CAR T cell function

This paper presents activation-inducible CAR T cells using CRISPR-mediated integration, enabling controlled CAR expression based on T cell activation status.

High-resolution profiling of neoantigen-specific T cell receptor activation signatures links moderate stimulation patterns to resilience and sustained tumor control

The paper identifies and characterizes neoantigen-specific T cell receptors (neoTCRs) using single-cell sequencing and in vitro/in vivo experiments, finding that moderate activation profiles enhance tumor control.

Deep Phenotyping and Lifetime Trajectories Reveal Limited Effects of Longevity Regulators on the Aging Process in C57BL/6J Mice

This study uses large-scale phenotyping in mice to show that longevity regulators affect age-sensitive phenotypes before detectable aging begins, rather than slowing aging rates.

Heritable changes in division speed accompany the diversification of singleT cell fate

This study tracks division speed and lineage fate in single CD8+ T cells during clonal expansion, revealing a burst phase of homogeneous division followed by diversification into effector and memory branches.

Spatial and temporal plasticity of neoantigen-specific T-cell responses bases on characteristics associated to antigen and TCR

The paper characterizes neoantigen-specific T-cell receptors in a melanoma patient, showing that low-avidity TCRs can provide effective anti-tumor responses and persist long-term in blood and tissues.

Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8+ Dendritic Cells

This study shows that Ppef2 deficiency in CD8+ dendritic cells leads to increased apoptosis and reduced antigen presentation, impacting T cell responses.

Systematic identification of cancer-specific MHC-binding peptides with RAVEN

The paper introduces RAVEN, a software tool that identifies cancer-specific genes encoding MHC-affine peptides from gene expression data, validated across 50 cancer types.