Author

E. Weerapana

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Microbiology· 3 Cancer Biology· 2 Cell Biology· 2

Signature Assays, Organisms & Methods

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Organism:human· 9 Assay:proteomics· 9 Approach:experimental assay· 7 Research Focus:cell type annotation· 5 Research Focus:drug response / drug discovery· 3 Approach:genetic perturbation / CRISPR· 2 Research Focus:protein folding & dynamics· 2 Research Focus:cancer & tumor microenvironment· 1

Frequent Co-Authors

D. W. Bak· 5 shared J. L. Meier· 3 shared W. M. Linehan· 3 shared K. Engelberg· 2 shared M. Perez· 2 shared M.-J. Gubbels· 2 shared S. E. Bergholtz· 2 shared T. Bechtel· 2 shared

Activity by Year

2018-2022 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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5 shared papersBiochemistry

D. W. Bak

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Author profile →

10 papers

Recent·Most cited

Conditional covalent lethality driven by oncometabolite accumulation

M. Perez, K. D. Nance, D. W. Bak, S. T. Gamage, S. S. Najera, A. N. Conte, W. M. Linehan, E. Weerapana, J. L. Meier

The paper identifies a covalent small molecule, MP-1, that exhibits FH-dependent cytotoxicity in HLRCC by targeting cysteine residues, including in TRMT1, and shows TRMT1 overexpression can rescue tRNA methylation inhibition.

bioRxiv · Apr 26, 2022Biochemistry

A more complex basal complex: novel components mapped to the Toxoplasma gondii cytokinesis machinery portray an expanded hierarchy of its assembly and function

K. Engelberg, T. Bechtel, C. Michaud, E. Weerapana, M.-J. Gubbels

This study maps the Toxoplasma gondii basal complex proteome and identifies new components, revealing a dynamic, hierarchical assembly process critical for cytokinesis.

bioRxiv · Oct 14, 2021 · PreprintMicrobiology

An infection-activated redox switch promotes tumor growth

Y. Kovalyova, D. W. Bak, E. M. Gordon, C. Fung, J. H. B. Shuman, T. L. Cover, M. R. Amieva, E. Weerapana, S. K. Hatzios

The paper shows that oxidation of legumain at Cys219 by H. pylori infection promotes tumor growth and mortality in a xenograft model.

bioRxiv · May 25, 2021 · PreprintMicrobiology

Monitoring iron-sulfur cluster occupancy across the E. coli proteome using chemoproteomics

D. W. Bak, E. Weerapana

This paper develops a chemoproteomic method to monitor Fe-S cluster occupancy in E. coli, identifying new Fe-S proteins and elucidating scaffold protein roles in Fe-S biogenesis.

bioRxiv · Apr 1, 2021 · PreprintBiochemistry

Prioritization of antimicrobial targets by CRISPR-based oligo recombineering

H. J. Benns, M. Storch, J. Falco, F. R. Fisher, E. Alves, C. J. Wincott, J. Baum, G. S. Baldwin, E. Weerapana, E. W. Tate, M. A. Child

This paper introduces CORe, a CRISPR-based method to prioritize reactive amino acids for drug discovery by linking sequence reactivity to protein function in Toxoplasma gondii.

bioRxiv · Feb 4, 2021Microbiology

Heterogeneous adaptation of cysteine reactivity to a covalent oncometabolite

M. Perez, D. W. Bak, S. E. Bergholtz, D. R. Crooks, Y. Yang, W. M. Linehan, E. Weerapana, J. L. Meier

The paper reports a chemoproteomic analysis of cysteine reactivity in HLRCC cells upon FH rescue, revealing transcriptional and posttranslational regulation of cysteine residues.

bioRxiv · Jun 25, 2020Cancer Biology

The antimalarial natural product salinipostin A identifies essential α/β serine hydrolases involved in lipid metabolism in P. falciparum parasites

E. Yoo, C. J. Schulze, B. H. Stokes, O. Onguka, T. Yeo, S. Mok, N. F. Gnadig, Y. Zhou, K. Kurita, I. T. Foe, S. M. Terrell, M. J. Boucher, P. Cieplak, R. G. Linington, J. Z. Long, , M. Bogyo

The study identifies essential α/β serine hydrolases in P. falciparum as targets of the antimalarial Salinipostin A, which disrupt lipid metabolism and inhibit parasite growth.

bioRxiv · Nov 1, 2019Cell Biology

The apical annuli of Toxoplasma gondii are composed of coiled-coil and signaling proteins embedded in the IMC sutures

K. Engelberg, C.-T. Chen, T. Bechtel, V. Sanchez Guzman, A. A. Drozda, S. Chavan, E. Weerapana, M.-J. Gubbels

This study identifies and characterizes apical annuli proteins in Toxoplasma gondii using BioID and SR-SIM, revealing their structural and functional roles in parasite replication and signaling.

Cellular Microbiology · Sep 10, 2019Peer-reviewedCell Biology

Generation of Recombinant Mammalian Selenoproteins through Genetic Code Expansion with Photocaged Selenocysteine

J. Peeler, R. Kelemen, M. Abo, L. Edinger, J. Chen, A. Chatterjee, E. Weerapana

This paper develops a method to generate and activate recombinant mammalian selenoproteins using genetic code expansion with photocaged selenocysteine, bypassing SECIS elements and selenium dependency.

bioRxiv · Sep 5, 2019Biochemistry

A chemoproteomic portrait of the oncometabolite fumarate

R. A. Kulkarni, D. W. Bak, D. Wei, S. E. Bergholtz, C. A. Briney, J. H. Shrimp, A. L. Thorpe, A. Bavari, A. Alpsoy, M. Levy, L. Florens, M. P. Washburn, E. C. Dykhuizen, N. Frizzell, E. Weerapana, , J. L. Meier

This study maps fumarate's covalent targets using chemoproteomic probes and identifies cysteine residues affected by fumarate or FH mutations in HLRCC cells.

bioRxiv · Mar 22, 2018Cancer Biology