E. W. Tate

Proximity proteomics reveals UCH-L1 as an NLRP3 interactor that modulates IL-1β production in human macrophages and microglia

The paper identifies UCH-L1 as an NLRP3 interactor that modulates IL-1β production in human macrophages and microglia through proximity proteomics and functional assays.

Inhibition of host N-myristoylation compromises the infectivity of SARS-CoV-2 due to Golgi-bypassing egress from lysosomes and endoplasmic reticulum

This study shows that inhibiting host N-myristoylation reduces SARS-CoV-2 infectivity by impairing viral envelope protein maturation and causing Golgi-bypassing egress.

Palmitoyl transferase ZDHHC20 promotes pancreatic cancer metastasis

This study identifies ZDHHC20 as a palmitoyl transferase promoting pancreatic cancer metastasis by affecting tumor-immune interactions, with no effect on proliferation or primary tumor growth.

Structural basis for membrane attack complex inhibition by CD59

This study reveals how CD59 inhibits MAC pore formation by binding C8 and deflecting C9, using cryo-EM structures and simulations.

XerC is required for the repair of antibiotic- and immune-mediated DNA damage in Staphylococcus aureus

This study identifies XerC as essential for DNA repair in Staphylococcus aureus, increasing susceptibility to antibiotics and immune defenses.

Inhibition of vaccinia virus L1 N-myristoylation by the host N-myristoyltransferase inhibitor IMP-1088 generates non-infectious virions defective in cell entry

This study shows that IMP-1088 inhibits vaccinia virus infection by blocking N-myristoylation of viral protein L1, leading to non-infectious virions.

Stable Flow-induced Expression of KLK10 Inhibits Endothelial Inflammation and Atherosclerosis

This study identifies KLK10 as a flow-sensitive endothelial protein that inhibits inflammation and atherosclerosis through a PAR1/2-dependent mechanism involving HTRA1 regulation.

Discovery of lipid-mediated protein-protein interactions in living cells using metabolic labeling with photoactivatable clickable probes

The paper develops lipid PTM-specific probes to capture protein-protein interactions in living cells, identifying novel interactors of myristoylated proteins.

Expression of mini-G proteins specifically halt cognate GPCR trafficking and intracellular signalling

This paper shows that mini-G proteins block GPCR trafficking and signaling by preventing receptor internalization, and proposes a nanobody-based alternative assay.

Structure and Mechanism of Hedgehog Acyl Transferase

The paper presents the cryo-EM structure of HHAT, revealing its mechanism for transferring palmitate to SHH and identifying a heme group essential for its function.

Plasmodium falciparum protein Pfs16 is a target for transmission-blocking antimalarial drug development

This study identifies Pfs16 as the cellular target of the N-4HCS compound DDD01035881, which blocks Plasmodium male gamete formation.

A novel tubulin binding molecule drives differentiation of acute myeloid leukaemia cells

The paper identifies a novel tubulin-binding molecule that induces differentiation in AML cells, reducing tumour burden and improving survival in vivo.

A suite of activity-based probes to dissect the KLK activome in drug-resistant prostate cancer

The paper develops activity-based probes to analyze KLK activome dynamics in prostate cancer, revealing KLK14's role in migration and secretion into the bone microenvironment.

N-myristoyltransferase inhibition is synthetic lethal in MYC-deregulated cancers

N-myristoyltransferase inhibition causes mitochondrial dysfunction and cell death in MYC-deregulated cancers through post-transcriptional failure in complex I synthesis.

Prioritization of antimicrobial targets by CRISPR-based oligo recombineering

This paper introduces CORe, a CRISPR-based method to prioritize reactive amino acids for drug discovery by linking sequence reactivity to protein function in Toxoplasma gondii.

Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress, and invasion

This study shows that inhibiting N-myristoylation in Plasmodium falciparum blocks parasite development, egress, and invasion through multiple mechanisms.

Photochemical probe identification of the small-molecule binding site in a mammalian membrane-bound O-acyltransferase

This study develops a photochemical probe to identify the small-molecule binding site in HHAT, leading to the discovery of a potent inhibitor and insights into MBOAT inhibition mechanisms.

Short chain fatty acids enhance expression and activity of the umami taste receptor in enteroendocrine cells via a Gαi/o pathway

This study shows SCFAs enhance umami receptor expression in EECs via Gi/o signaling, improving nutrient sensing without affecting SCFA GPCRs.

Endosomal free fatty acid receptor 2 signaling is essential for propionate-induced anorectic gut hormone release

This study shows propionate activates endosomal Gi/p38 signaling via FFA2 to induce GLP-1 release in enteroendocrine cells.

Wheat pathogen Zymoseptoria tritici N-myristoyltransferase inhibitors: on-target antifungal activity and an unusual metabolic defense mechanism

This study identifies N-myristoyltransferase inhibitors for the wheat pathogen Zymoseptoria tritici and reveals a novel metabolic defense mechanism through proteomic and bioinformatics analysis.

Targeting STAT3 signalling using stabilised sulforaphane (SFX-01) inhibits endocrine resistant stem-like cells in ER-positive breast cancer

This study shows that SFX-01 reduces CSC activity in ER+ breast cancer by inhibiting STAT3 signaling, improving outcomes in endocrine-resistant models.

Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins.

The paper shows Rab27a recruits SPIRE1/2 to melanosomes, linking motor and track assembly proteins to enable long-range organelle transport via actin tracks.