J. L. Meier

Paralogue-selective degradation of the lysine acetyltransferase EP300

This study develops a small molecule, MC-1, that selectively degrades EP300 over CREBBP, revealing factors governing paralogue-specific degradation and its impact on cancer cell proliferation.

Molecular Basis for RNA Cytidine Acetylation by NAT10

This study determines the cryo-EM structure of NAT10 bound to a cytidine-CoA probe, revealing its mechanism for RNA N4-cytidine acetylation and its role in cellular processes.

Loss of NAT10 disrupts enhancer organization via p300 mislocalization and suppresses transcription of genes necessary for metastasis progression.

This study shows that NAT10 loss reduces lung metastasis by disrupting enhancer organization through p300 mislocalization and altering gene transcription related to metastasis.

Comparative analysis of drug-like EP300/CREBBP acetyltransferase inhibitors

This study compares the biochemical and biological potencies of three EP300/CREBBP acetyltransferase inhibitors and demonstrates their utility in investigating epigenetic mechanisms.

Chemoproteomics yields a selective molecular host for acetyl-CoA

The paper develops a chemoproteomics-based method to selectively detect acetyl-CoA using a Nanoluc-NAA50 fusion protein and a ligand displacement assay.

Reference compounds for characterizing cellular injury in high-content cellular morphology assays

The paper profiles 218 cytotoxic and nuisance compounds in U-2 OS cells to create reference compounds for improving assay optimization and compound prioritization in cellular morphology assays.

Antisense pairing and SNORD13 structure guide RNA cytidine acetylation

This study identifies molecular mechanisms of RNA cytidine acetylation by SNORD13 and NAT10 using mutational analysis and ac4C sequencing in human cells.

Conditional covalent lethality driven by oncometabolite accumulation

The paper identifies a covalent small molecule, MP-1, that exhibits FH-dependent cytotoxicity in HLRCC by targeting cysteine residues, including in TRMT1, and shows TRMT1 overexpression can rescue tRNA methylation inhibition.

Protonation-Dependent Sequencing of 5-Formylcytidine in RNA

This paper develops a protonation-dependent sequencing method to detect 5-formylcytidine and 5-carboxycytidine in RNA, demonstrating its application in human tRNA and a 5fC-deficiency model.

Site-Specific Synthesis of N4-Acetylcytidine in RNA Reveals Physiological Duplex Stabilization

This study synthesizes and characterizes N4-acetylcytidine (ac4C) in RNA to assess its impact on duplex stability and mismatch discrimination in eukaryotic sequences.

Harnessing Ionic Selectivity In Acetyltransferase Chemoproteomic Probes

The paper develops a CoA-based chemoproteomic strategy to optimize probe selectivity and evaluates its application in assessing small molecule acetyltransferase inhibitors.

Synthesis and Evaluation of a Stable Isostere of Malonyllysine

This study synthesizes and evaluates a stable isostere of malonyllysine, demonstrating its resistance to decarboxylation and antibody recognition.

Heterogeneous adaptation of cysteine reactivity to a covalent oncometabolite

The paper reports a chemoproteomic analysis of cysteine reactivity in HLRCC cells upon FH rescue, revealing transcriptional and posttranslational regulation of cysteine residues.

Direct anabolic three carbon metabolism via propionate to a six carbon metabolite occurs in human heart and in vivo across mouse tissues

The paper confirms the in vivo synthesis of a six-carbon metabolite from propionate in human heart and mouse tissues through non-oxidative pathways.

Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues

This paper quantifies lactoyl-CoA in mammalian cells and tissues using LC-HRMS and provides a biosynthetic method for isotopically labeled lactoyl-CoA.

An Oncometabolite Isomer Rapidly Induces A Pathophysiological Protein Modification

This study develops chemical agonists to induce S-succination, a posttranslational modification linked to oncometabolites, and demonstrates their cellular effects.

A systems chemoproteomic analysis of acyl-CoA/protein interactions

The paper develops a chemoproteomic platform to analyze acyl-CoA/protein interactions and identifies biological processes affected by acetyl-CoA levels.

A chemoproteomic portrait of the oncometabolite fumarate

This study maps fumarate's covalent targets using chemoproteomic probes and identifies cysteine residues affected by fumarate or FH mutations in HLRCC cells.

Chemical control of a CRISPR-Cas9 acetyltransferase

The paper develops a CRISPR-Cas9-based tool to study acetylation-dependent gene expression and identifies small molecule inhibitors of p300 activity.

Bioorthogonal pro-metabolites for profiling short chain fatty acylation

This paper introduces bioorthogonal pro-metabolites to profile short chain fatty acylation in cells, identifying novel protein targets and characterizing their metabolic and enzymatic effects.