K. A. Verba

High-resolution single-particle imaging at 100-200 keV with the Gatan Alpine direct electron detector.

This study evaluates the Gatan Alpine detector at 100-200 keV for cryo-EM, showing improved resolution and performance compared to the Gatan K3.

Structural dynamics of the active HER4 and HER2/HER4 complexes is finely tuned by different growth factors and glycosylation

This study reports cryo-EM structures of HER2/HER4 heterodimers and HER4 homodimers bound to different ligands, revealing structural dynamics and glycan contributions to receptor activation.

Structural insights into regulation of the PEAK3 scaffold by 14-3-3

This paper reports the cryo-EM structure of PEAK3 pseudokinase in complex with 14-3-3, revealing an asymmetric binding mechanism and identifying Protein Kinase D as a regulator.

Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor traps

The paper develops a computational pipeline combining Rosetta and cryo-EM to analyze ACE2 receptor traps' binding to SARS-CoV-2 variants, showing high affinity and neutralization capabilities against Omicron and Delta.

Structure-function analysis of enterovirus protease 2A in complex with its essential host factor SETD3

The paper reports the cryo-EM structure of SETD3 interacting with enterovirus protease 2A and shows that mutations in the SET domain reduce binding and protect against infection.

Evolution of enhanced innate immune evasion by the SARS-CoV-2 B.1.1.7 UK variant

This study identifies that the B.1.1.7 SARS-CoV-2 variant enhances innate immune evasion through increased Orf9b and Orf6 expression and activity.

Structures of the active HER2/HER3 receptor complex reveal dynamics at the dimerization interface induced by binding of a single ligand

This study presents cryo-EM structures of the HER2/HER3 receptor complex bound to NRG1β, revealing conformational dynamics at the dimerization interface and how mutations and therapeutics interact with this interface.

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

This study optimizes K3 camera settings in CDS mode for cryo-EM, showing improved resolution and throughput for protein complex imaging.