K. Hattori

Heart generation via blastocyst complementation in Mesp1/2-deficient mice

This study generates functional adult hearts in Mesp1/2-deficient mice using blastocyst complementation with pluripotent stem cells, showing successful derivation of all cardiovascular lineages from exogenous cells.

Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis/release regulators

The paper introduces CIBER, a CRISPR-based method to barcode sEVs for subpopulation-specific analysis of their release regulators, identifying new regulators and characterizing sEV biogenesis.

A novel kit mutant rat enables hematopoietic stem cell engraftment without irradiation

This study introduces a KitV834M mutant rat model that supports HSC engraftment without irradiation, offering a larger-scale alternative to mouse models for HSC research.

High-throughput 3D imaging flow cytometry of adherent 3D cell cultures

This paper develops a high-throughput 3D imaging flow cytometry platform for analyzing spheroid models on hydrogel beads, revealing adhering cells have smaller nuclei with non-round surfaces.

A bispecific antibody NXT007 exerts a hemostatic activity in hemophilia A monkeys enough to keep a non-hemophiliac state

This paper reports a bispecific antibody NXT007 that shows improved hemostatic activity in hemophilia A monkeys, equivalent to FVIII levels, with lower dosing requirements and longer intervals.

Droplet array-based platform for parallel optical analysis of dynamic extracellular vesicle secretion from single cells

This study develops a droplet array platform to track single-cell extracellular vesicle secretion dynamics over time, revealing heterogeneity and the role of cell division in EV secretion.

Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium

This study identifies Semaphorin 3G as a factor that worsens joint inflammation by promoting macrophage accumulation and proliferation in RA synovium.

Immunological features that determine the strength of antibody responses to BNT162b2 mRNA vaccine against SARS-CoV-2

The paper identifies immunological features, including B cell frequencies and CD8+ T cell activation, that correlate with antibody response strength to the BNT162b2 mRNA vaccine.

High-throughput organoid screening enables engineering of intestinal epithelial composition

This paper develops a high-throughput organoid screening method to identify agents that alter intestinal epithelial composition, finding Xpo1 inhibitors increase Paneth cell abundance.