O. Medalia

Cryo-electron tomography reveals the nanoscale architecture of the actin cortex in cellular blebs

This study uses cryo-ET to reveal the nanoscale 3D organization of cortical actin in cellular blebs, showing short and straight actin filaments and structural parameters.

Delayed localization of A-type lamins to the rupture sites in Hutchinson-Gilford progeria syndrome

This study investigates the delayed localization of A-type lamins to nuclear envelope rupture sites in HGPS, identifying farnesylation and proteolytic cleavage as key factors affecting this process.

Vimentin filaments integrate low complexity domains in a highly complex helical structure

This study determines the 3D structure of vimentin intermediate filaments, revealing how disordered domains contribute to their mechanical properties.

Keratin isoform shifts modulate motility signals during wound healing

The paper shows that keratin isoform shifts during wound healing modulate motility signals by activating myosin motors through isoform-specific interactions with vimentin shuttling kinases.

A link between genotype and cellular architecture in microbiome members as revealed by cryo-EM

The paper uses cryo-EM to analyze microbial cellular architecture and finds that structural similarity correlates with taxonomic distance within families but not above.

A network of mixed actin polarity in the leading edge of spreading cells

This study uses cryo-ET to reveal three distinct actin sub-domains in lamellipodia, including filaments with barbed ends pointing toward the cell center.

How talin allosterically activates vinculin

This study identifies an allosteric network in vinculin regulated by talin, showing how force and binding dynamics control focal adhesion formation.

Chiral growth of adherent filopodia

This study identifies myosin-X and DAAM1 as key factors promoting chiral growth in adherent filopodia, revealing how actin dynamics drive left-right asymmetry in cellular protrusions.

An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in S. cerevisiae

This study identifies Brl1's amphipathic helix as essential for NE fusion during NPC biogenesis in yeast.

Vimentin Intermediate Filaments and Filamentous Actin Form Unexpected Interpenetrating Networks That Redefine the Cell Cortex

This study reveals that vimentin intermediate filaments and F-actin form an interpenetrating network in the cell cortex, enhancing contractile forces and influencing actin dynamics.

Structural basis for the activation and ligand recognition of the human oxytocin receptor

This study determines the cryo-EM structure of the oxytocin receptor bound to oxytocin, revealing its activation mechanism and ligand recognition.

Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS

The paper shows lamin C, BAF, and cGAS accumulate at nuclear envelope rupture sites in mouse cells, with lamin A/C involvement in DNA sensing.

The molecular architecture of vimentin filaments

This study reveals the subnanometer structure of vimentin filaments in mouse cells, showing their helical symmetry and molecular composition.

Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy

This study uses cryo-electron microscopy to reveal the structural heterogeneity of keratin 5/keratin 14 filaments, showing variations in diameter and subunit organization.

Revealing the polarity of actin filaments by cryo-electron tomography

This study develops a method to determine actin filament polarity in cryo-tomograms and shows a linear relationship between actin polarity and distance from the apical boundary in focal adhesions.

Nanoscale resolution of microbial fiber degradation in action

This study reveals the in-situ organization of cellulosomes and their substrates during microbial fiber degradation, showing phenotypic heterogeneity and growth-phase dependent dynamics.

A lamin A/C variant causing striated muscle disease provides insights into filament organization

This study examines a lamin A/C mutation in mice, revealing structural changes in nuclear filaments and chromatin organization linked to striated muscle disease.

Computational analysis of lamin isoform interactions with nuclear pore complexes

The paper analyzes lamin isoform interactions with nuclear pore complexes using microscopy data from mouse fibroblasts, showing their role in maintaining nuclear envelope organization.

Nonlinear mechanics of lamin filaments and the meshwork topology build an emergent nuclear lamina

The paper measures the mechanical properties of lamin filaments in their native meshwork using multiple techniques and finds they exhibit non-Hookean behavior and small-world network topology.

Morphometric analysis of spread platelets identifies integrin αIIbβ3-specific contractile phenotype

The paper develops a morphometric screen to analyze platelet cytoskeletal organization and identifies integrin αIIbβ3-specific contractile phenotypes in response to matrix stiffness and inhibitors.

Conformational states during vinculin unlocking differentially regulate focal adhesion properties

This study investigates how vinculin's conformational changes affect focal adhesion properties by analyzing the transition from closed to open states.