L. C. Kapitein

Aurora B controls microtubule stability to regulate abscission dynamics in stem cells

The paper shows Aurora B regulates abscission speed in stem cells by controlling microtubule stability through a feedback loop.

GelMap: Intrinsic calibration and deformation mapping for expansion microscopy

GelMap introduces a fluorescent grid to measure expansion factors and deformation in expansion microscopy, enabling precise quality control and sample navigation.

Nuclear poly-glutamine aggregates rupture the nuclear envelope and hinder its repair

Nuclear poly-glutamine aggregates cause nuclear envelope ruptures that impair repair, contributing to Huntington's disease pathology.

Direct observation of motor protein stepping in living cells using MINFLUX

This study uses MINFLUX to track motor protein stepping in living cells and resolve their spatial organization on microtubules.

The SARS-CoV-2 accessory factor ORF7a downregulates MHC class I surface expression

This study identifies SARS-CoV-2 ORF7a as a viral factor that downregulates MHC class I surface expression, impairing antigen presentation and immune surveillance.

Opto-Katanin: An Optogenetic Tool for Localized Microtubule Disassembly

This paper introduces opto-katanin, a light-activated tool for localized microtubule disassembly, enabling precise spatiotemporal control in mammalian cells.

Self-assembly of pericentriolar material in interphase cells lacking centrioles

This study shows that PCM can self-assemble into a compact MTOC in interphase cells without centrioles, using dynein transport and self-clustering, but this process is sensitive to motor and microtubule dynamics.

Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia

This study develops an improved method to measure CFTR drug responses in nasal airway organoids derived from 2D differentiated epithelial cells.

A live-cell marker to visualize the dynamics of stable microtubules

This paper introduces a live-cell marker to visualize stable microtubules and demonstrates its dynamics in living cells.

Lattice defects induced by microtubule-stabilizing agents exert a long-range effect on microtubule growth by promoting catastrophes

This study shows that microtubule-stabilizing agents induce lattice defects that cause long-range catastrophes in microtubule growth.

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)

This paper introduces TREx, a modified expansion microscopy method that achieves ten-fold sample expansion without specialized equipment, enabling high-resolution imaging of both thick tissue sections and cultured cells.

Kinesin-4 KIF21B limits microtubule growth to allow rapid centrosome polarization in T cells.

This study shows that KIF21B limits microtubule growth to enable rapid centrosome polarization in T cells by inducing microtubule pausing and catastrophe.

REALM: AO-based localization microscopy deep in complex tissue

REALM improves single-molecule localization in complex tissues by correcting aberrations using adaptive optics and blinking molecules.

Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

The paper shows that KIF5B and KIF13B work together to transport secretory vesicles to microtubule plus ends, with KIF5B playing a dominant role and KIF13B assisting in reaching newly polymerized ends.

Mechanisms of motor-independent membrane remodeling driven by dynamic microtubules

This study reconstitutes membrane tubulation using dynamic microtubules and proteins, revealing three motor-independent mechanisms for membrane remodeling.

MAP7 family proteins regulate kinesin-1 recruitment and activation

The paper shows that MAP7 family proteins activate kinesin-1 by enhancing its microtubule binding and processivity through transient interactions.

Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots

This study uses quantum dots to investigate how cytoskeletal structures affect intracellular transport dynamics, revealing actin's role in suppressing microtubule remodeling and influencing diffusion rates.