T. van Opijnen

Evaluation of the fitness benefit conferred by RNA cis-regulators to Streptococcus pneumoniae during infection

This study evaluates the fitness benefit of RNA cis-regulators in Streptococcus pneumoniae during infection, finding that mis-regulation significantly reduces fitness in vivo.

Identification of Determinants that Allow Maintenance of High-Level Fluoroquinolone Resistance in Acinetobacter baumannii

The paper identifies proteins essential for maintaining fitness in Acinetobacter baumannii strains overexpressing RND efflux pumps, highlighting the role of outer membrane integrity and compensatory mechanisms.

Dual Membrane-Sspanning Anti-Sigma Factors Regulate Vesiculation in Gut Bacteroidota

This study identifies a novel family of Dual Membrane-spanning Anti-sigma factors (Dma) that regulate Outer Membrane Vesicle (OMV) biogenesis in Bacteroides thetaiotaomicron.

Genome-wide phage susceptibility analysis in Acinetobacter baumannii reveals capsule modulation strategies that determine phage infectivity

This study identifies capsule modulation strategies in Acinetobacter baumannii that determine phage infectivity using Tn-seq and genetic analysis.

A bacterial pan-genome makes gene essentiality strain-dependent and evolvable

The paper investigates how a bacterial pan-genome affects gene essentiality and shows that essential genes can evolve to become non-essential through genetic changes and mechanisms like functional redundancy and pathway rewiring.

A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance.

This study creates a genome-wide atlas of antibiotic susceptibility targets in Streptococcus pneumoniae, identifying a 'tolerome' that influences resistance and highlights its evolutionary significance.

Yersinia pseudotuberculosis doxycycline tolerance strategies include modulating expression of genes involved in cell permeability and tRNA modifications

This study identifies gene expression changes in Yersinia pseudotuberculosis in response to doxycycline, showing that OmpF porin and TusB tRNA modification influence antibiotic tolerance.

Rapid, multianalyte detection of opioid metabolites in wastewater

This paper presents an aptamer-based graphene field effect transistor platform for rapid, reliable, and cost-effective detection of three opioid metabolites in wastewater.

Immunosuppression broadens evolutionary pathways to treatment failure during Acinetobacter baumannii pneumonia

This study shows that immunosuppression promotes antibiotic resistance evolution in Acinetobacter baumannii pneumonia by enabling persistence mutations that cause treatment failure without clinical resistance.

Dynamic pneumococcal genetic adaptations support bacterial growth and inflammation during coinfection with influenza

This study identifies bacterial genes critical for pneumococcal virulence and immune modulation during influenza coinfection using transposon mutagenesis and in vivo analysis.

Essential gene analysis in Acinetobacter baumannii by high-density transposon mutagenesis and CRISPR interference

This study identifies essential genes in Acinetobacter baumannii using transposon mutagenesis and a CRISPRi system, revealing new factors and conditionally essential processes.

Detection of a multi-disease biomarker in Salivawith Graphene Field Effect Transistors

This paper develops a label-free biosensor using graphene field effect transistors to detect CA1 in saliva, achieving high sensitivity and clinical relevance.

Topologically correct synthetic reconstruction of pathogen social behavior found in deep tissue sites

The paper reconstructs microbial social behavior in deep tissue sites using microfluidics-driven microdroplets and NO generation, mimicking spatial regulation observed in tissues.

Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs

This paper presents a biosensor using G-FETs and dielectrophoresis to rapidly and selectively detect antibiotic-resistant bacteria at the single-cell level.

The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence

droplet-Tn-Seq combines microfluidics with Tn-Seq identifying complex single-cell phenotypes

droplet-Tn-Seq uses microfluidics to encapsulate transposon mutants individually, enabling single-cell analysis of bacterial fitness while retaining Tn-Seq advantages.