W. Huo

Massively parallel combination screen reveals small molecule sensitization of antibiotic-resistant Gram-negative ESKAPE pathogens

This study uses high-throughput screening to identify small molecules that enhance antibiotic efficacy against multidrug-resistant Gram-negative bacteria.

Loss of essential outer membrane functions causes drug hypersensitization in Acinetobacter baumannii overexpressing multidrug efflux pumps

The paper identifies essential genes in Acinetobacter baumannii that support efflux pump overproducer fitness during ciprofloxacin treatment, revealing roles in outer membrane integrity and pH homeostasis.

Identification of Determinants that Allow Maintenance of High-Level Fluoroquinolone Resistance in Acinetobacter baumannii

The paper identifies proteins essential for maintaining fitness in Acinetobacter baumannii strains overexpressing RND efflux pumps, highlighting the role of outer membrane integrity and compensatory mechanisms.

Enterococcus faecalis strains with compromised CRISPR-Cas defense emerge under antibiotic selection for a CRISPR-targeted plasmid

This study shows that antibiotic selection leads to compromised CRISPR-Cas defense in E. faecalis, enabling enhanced acquisition of antibiotic resistance plasmids.

Inhibition of the DENV2 and ZIKV RNA polymerases by Galidesivir triphosphate measured using a continuous fluorescence assay

This paper measures the inhibition of DENV2 and ZIKV RNA polymerases by Galidesivir triphosphate using a continuous fluorescence assay, finding it equipotent against both with IC50 values of 42 and 47 M.

5' Untranslated mRNA Regions Allow Bypass of Host Cell Translation Inhibition by Legionella pneumophila

This study identifies host proteins selectively synthesized during Legionella pneumophila infection and shows that 5' untranslated regions (5UTRs) enable translation of low-abundance transcripts despite host translation inhibition.

Immunosuppression broadens evolutionary pathways to treatment failure during Acinetobacter baumannii pneumonia

This study shows that immunosuppression promotes antibiotic resistance evolution in Acinetobacter baumannii pneumonia by enabling persistence mutations that cause treatment failure without clinical resistance.

Arabidopsis root responses to salinity depend on pectin modification and cell wall sensing

This study investigates how Arabidopsis root responses to salinity depend on pectin modification and cell wall sensing, showing that FER/HERK1/THE1 and PME activity influence stress signaling and gene expression.

Reconstruction of Bone Defect Based on the Fusion of Osteoblasts and MSM/HA/PLGA Porous Scaffolds

This study develops and evaluates porous scaffolds combining HA, PLGA, and MSM for bone defect reconstruction, showing improved cell adhesion, proliferation, and drug release properties.

Chromosomal Resistance to Metronidazole in Clostridioides difficile can be Mediated By Epistasis Between Iron Homeostasis and Oxidoreductases

This study identifies multi-genetic mechanisms of metronidazole resistance in C. difficile, showing epistasis between iron homeostasis and oxidoreductases.

A Type I Restriction-Modification System Associated with Enterococcus faecium Subspecies Separation

The paper identifies a Type I restriction-modification system in Clade A1 E. faecium that reduces transformability and contributes to subspecies separation.

Loss of function mutations in epaR confer resistance to phage NPV1 infection in Enterococcus faecalis OG1RF

This study identifies loss-of-function mutations in epaR as conferring resistance to phage NPV1 in E. faecalis OG1RF by blocking phage adsorption, but these mutations also increase susceptibility to daptomycin and osmotic stress.