T. K. Hughes

Protective intravenous BCG vaccination induces enhanced immune signaling in the airways

This study shows that high-dose IV BCG vaccination in rhesus macaques enhances airway immune signaling and cellular responses, improving protection against Mtb.

Gene signatures and host-parasite interactions revealed by dual single-cell profiling of Plasmodium vivax liver infection

This study uses single-cell profiling to analyze P. vivax liver infection, identifying host and parasite transcriptional signatures and revealing new insights into parasite dormancy and host responses.

Single Cell and Spatial Transcriptomics Defines the Cellular Architecture of the Antimicrobial Response Network in Human Leprosy Granulomas

The paper uses single cell and spatial transcriptomics to map the cellular architecture of antimicrobial responses in leprosy granulomas, identifying gene expression differences and spatial organization in reversal reactions.

Multimodal profiling of lung granulomas reveals cellular correlates of tuberculosis control

The paper identifies cellular and temporal factors associated with bacterial control in tuberculosis granulomas using multimodal data.

Single-cell profiling of Ebola virus infection in vivo reveals viral and host transcriptional dynamics

This study uses single-cell transcriptomics and protein profiling to analyze EBOV infection in rhesus monkeys, revealing changes in immune cell dynamics and viral/host gene expression.

Highly Efficient, Massively-Parallel Single-Cell RNA-Seq Reveals Cellular States and Molecular Features of Human Skin Pathology

The paper introduces an improved scRNA-Seq protocol, Seq-Well S^3, which enhances transcript capture and gene detection efficiency, and applies it to study inflammatory skin diseases.

Reduced cellular diversity and an altered basal progenitor cell state inform epithelial barrier dysfunction in human type 2 immunity

This study profiles single-cell RNA-seq data from human respiratory tissues to show reduced epithelial diversity and altered basal progenitor states in type 2 immunity-driven diseases.