V. Viasnoff

Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy

This paper accelerates fluorescence lifetime imaging (FLIM) using single-objective light-sheet microscopy, achieving faster and less phototoxic imaging of live multicellular systems.

E-cadherin-dependent phosphorylation of EGFR governs a homeostatic feedback loop controlling intercellular junction viscosity and collective migration modes.

Cortical ductility governs cell-cell adhesion mechanics

This study introduces a new method to measure energy dissipation during cell-cell junction rupture and shows that cortical tension and shape relaxation time determine whether junctions behave ductile or brittle.

Selective volumetric excitation and imaging for single molecule localization microscopy in multicellular systems

This paper introduces a method for volumetric excitation and 4D imaging of single molecules in multicellular systems using light sheet and multifocus microscopy.

Crosstalk between myosin II and formin functions in the regulation of force generation and actomyosin dynamics in stress fibers

The paper measures traction forces and actomyosin dynamics in stress fibers, revealing that actin turnover and traction forces depend on both myosin II and formin activities.

ECM dimensionality tunes actin tension to modulate the endoplasmic reticulum and spheroid phenotype

The paper shows that ECM dimensionality affects actin tension, which in turn modulates endoplasmic reticulum function and spheroid resilience in mammary epithelial cells.

Cooperative regulation of adherens junction elongation through Epidermal Growth Factor Receptor (EGFR) activation.

The paper shows that EGFR activation increases adherens junction elongation speed without affecting final size in suspended cell aggregates.

High content 3D imaging method for quantitative characterization of organoid development and phenotype.

The paper introduces a high-content 3D imaging platform for organoid development analysis, enabling automated, high-throughput imaging and AI-based quantification of morphological features across multiple scales.

The Formin Inhibitor, SMIFH2, Inhibits Members of the Myosin Superfamily

The paper shows SMIFH2 inhibits myosin ATPase activity and actin filament translocation, affecting myosin 2A, myosin 10, and other non-muscle myosins, suggesting caution in using SMIFH2 to infer formin function.

A Method to sort heterogenous cell populations based on migration in 2D and 3D environments

This paper introduces a method to sort migratory cancer and immune cells based on their spontaneous migration in 2D and 3D environments, revealing enhanced cytotoxicity in enriched migratory cytotoxic T lymphocytes.

Extracellular domains of E-cadherin determine key mechanical phenotypes of an epithelium through cell- and non-cell-autonomous outside-in signalling

The paper investigates how extracellular domains of E-cadherin influence epithelial mechanical properties through cell- and non-cell-autonomous signaling.

Autonomous induction of hepatic polarity to construct single cell liver

This study shows that mature hepatocytes can autonomously polarize to form secretory hemi-apical lumens on bio-functionalized substrates, enabling controlled study of lumenogenesis.

Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity

The paper develops a single-cell assay to analyze EphA receptor clustering in cancer cells, revealing its persistence through division and association with migration and drug resistance.

Forces and constraints controlling podosome assembly and disassembly

This study investigates how mechanical forces and membrane tension affect podosome assembly and disassembly in cells.

Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement

This study examines actin cytoskeleton self-organization in fibroblasts and epitheliocytes under isotropic confinement, revealing differences in pattern progression and the role of keratin networks.

Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets.

This study uses mechanical stimulation of suspended cell doublets to investigate how cortical actin and junction proteins respond to compression versus stretch at cell-cell contacts.

Physics of lumen growth

The paper models the dynamics of intercellular secretory lumen formation, predicting a critical pumping threshold and a transition between monotonous and oscillatory regimes during luminogenesis.

A new approach to design artificial 3D micro-niches with combined chemical, topographical and rheological cues

This paper introduces a method to create 3D micro-niches with combined chemical, topographical, and rheological cues to study cell behavior.