Y. Ohkawa

Importin α Characterizes a Micronuclear Environment Associated with Genomic Instability in Human Cancer Cells

The paper identifies importin α as a marker of a distinct micronuclear environment in cancer cells, associated with genomic instability and immune evasion.

Precise immunofluorescence canceling for highly multiplexed imaging capturing specific cell state

The paper introduces PECAbs for high-specificity multiplexed imaging of cell states and combines them with seq-smFISH to classify cells and identify signal activation states in human tissue.

Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by single-cell transcriptomics

This study identifies Atf3 as a key regulator of transition from compensation to dysfunction in female mitochondrial cardiomyopathy using single-cell transcriptomics.

The anti-tumor effect of trifluridine via induction of aberrant mitosis is unaffected by mutations modulating p53 activity

This study shows that trifluridine induces aberrant mitosis and cytotoxicity in both p53-null and p53-GOF mutant cells, independent of p53 function.

Metabolic clogging of mannose triggers genomic instability via dNTP loss in human cancer cells

This study shows that excess mannose causes dNTP depletion, leading to genomic instability and enhanced sensitivity to chemotherapy in cancer cells.

Phase-separated nuclear bodies of nucleoporin fusions, SET-NUP214 and NUP98-HOXA9, promote condensation of MLL1 and CRM1 to activate target genes

This study shows that nucleoporin fusions form phase-separated nuclear bodies that enhance gene activation by condensing MLL1 and CRM1 at target loci.

The ulcerative colitis associated gene FUT8 regulates the quantity and quality of secreted mucins

This study shows that FUT8 regulates mucin secretion and viscoelastic properties, linking its dysfunction to ulcerative colitis pathogenesis.

Chromatin loading of MCM hexamers is associated with di-/tri-methylation of histone H4K20 toward S phase entry

This study investigates MCM hexamer states during the cell cycle in human cells and links their formation to histone H4K20 methylation levels, showing that di/trimethyl-H4K20 correlates with double MCM hexamer formation.

Visualizing transcription sites in living cells using a genetically encoded probe specific for the elongating form of RNA polymerase II

The paper develops a genetically encoded probe to detect elongating RNA polymerase II in living cells and shows its foci move more dynamically than chromatin domains.

Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay

The paper develops a method to study histone variant incorporation and finds that chromatin structure and DNA replication influence histone deposition patterns.

Genomic profiling of PML bodies reveals transcriptional regulation by PML bodies through the DNMT3A exclusion

The paper identifies genomic regions associated with PML bodies and shows their role in maintaining hypo-methylated states at Y-linked gene promoters through DNMT3A exclusion.

Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning

The paper shows that cell competition corrects noisy Wnt/β-catenin gradients by eliminating unfit cells, ensuring robust embryonic patterning.