Takashi Ito

High extracellular osmolarity promotes yeast thermotolerance through osmotic modulation and glycerol-dependent adaptation.

This study shows that high extracellular osmolarity improves yeast thermotolerance by modulating osmotic balance and enhancing glycerol accumulation under heat stress.

Sox4 in Treg Cells Suppresses IL-10 Production via c-Maf Degradation and Exacerbates Type 2 Inflammation

This study identifies Sox4 as a suppressor of IL-10 production in Treg cells by degrading c-Maf, leading to exacerbated type 2 inflammation in asthma models.

LOBSTERS: a modular vector series with a diverse set of Cas nucleases and plasmid selection markers for multiplex genome editing in Saccharomyces cerevisiae

LOBSTERS provides a modular CRISPR system with diverse Cas nucleases and plasmid markers for efficient multiplex genome editing in yeast.

DNA methylation protects cancer cells against senescence

The paper shows that reduced DNA methylation in cancer cells leads to senescence without DNA damage, involving p21 and cGAS, and demonstrates this in vivo.

Strategic targeting of Cas9 nickase expands tandem gene arrays

The paper introduces BITREx, a method using Cas9 nickase to expand tandem gene arrays in yeast and mammalian cells.

Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells

This study shows UHRF1 has non-canonical roles beyond DNMT1 activation in maintaining DNA methylation homeostasis in cancer cells.

A genome-wide knock-out screen for actors of epigenetic silencing reveals new regulators of germline genes and 2-cell like cell state

The paper identifies new regulators of germline genes and 2-cell like cell state through a genome-wide CRISPR knock-out screen in mouse ES cells.

Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae

The paper introduces a unified set of CRISPR/Cas vectors and software for efficient genome editing in yeast using SpCas9, SaCas9, and AsCas12a.

Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle

This study identifies Hoxa10 as a mediator of positional memory in adult skeletal muscle stem cells, linking embryonic gene expression to region-specific regenerative capacity.

Short single-stranded DNA with putative non-canonical structures comprises a novel class of plasma cell-free DNA

The paper identifies short single-stranded DNA fragments in plasma that form non-canonical structures and are enriched in open chromatin and transcription factor-binding sites.