Massimiliano Clamer

Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systems

The paper examines the translation efficiency of synthetic circRNAs using different IRESes in human cells and in vitro systems, finding that several IRESes effectively drive circRNA translation.

Selective profiling of translationally active tRNAs and their dynamics under stress

The paper introduces tRIBO-seq, a nanopore-based method to profile ribosome-associated tRNAs, revealing stress-induced changes in tRNA abundance, modification, and fragmentation.

Empowering multiplexed ultra-throughout ribosome profiling with RiboWich

RiboWich improves ribosome profiling by eliminating ribosome purification and enabling high-throughput, low-input translatome analysis.

Disruption of ribosome dynamics and mRNA homeostasis triggers a cascading imbalance in protein synthesis in models of Amyotrophic Lateral Sclerosis

This study investigates how TDP-43 dysregulation disrupts protein synthesis in ALS models by altering ribosome dynamics and mRNA homeostasis, revealing a conserved mechanism across in vitro and in vivo systems.

Phospho-RNA sequencing with CircAID-p-seq

The paper introduces circAID-p-seq, a PCR-free method for phospho-RNA sequencing, and demonstrates its application in ribosome profiling to study RNA-protein interactions.

A specialized mRNA translation circuit instated in pluripotency presets the competence for cardiogenesis in humans

The paper shows that RBPMS controls mRNA translation in pluripotent hESCs to preset cardiac fate competence, which is critical for cardiogenesis.

SMN-primed ribosomes modulate the translation of transcripts related to Spinal Muscular Atrophy

The paper shows SMN protein binds ribosomes and modulates translation of specific mRNAs in SMA, causing translational defects in motor neuron-related proteins.

Active ribosome profiling with RiboLace

The paper introduces RiboLace, a method to isolate active ribosomes for ribosome profiling, enabling high-resolution analysis of active translation.