Ashwin N. Skelly

Early clonal dominance at priming sets the trajectory for broad HIV serum neutralization

The paper shows that early clonal dominance in B-cell precursors after vaccination predicts the breadth of HIV-neutralizing antibodies in rhesus macaques.

Rapid vaccine induction of macaque HIV-1 V2 Apex broadly neutralizing antibodies with immunogenetic signatures that are potentially translatable to humans

This study shows that vaccination of macaques with an Env-ferritin nanoparticle rapidly elicits V2-apex broadly neutralizing antibodies with immunogenetic features that may be applicable to humans.

Deep Mining of the Human Antibody Repertoire Identifies Frequent and Genetically Diverse CDRH3 Topologies Targetable by Vaccination

The paper identifies frequent and genetically diverse CDRH3 topologies in the human antibody repertoire that can be targeted by vaccination to elicit broadly neutralizing antibodies.

Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques

This study shows that a germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaques by priming V2-apex bnAb B cell precursors.

Shared Maturation Pathways of HIV-1 Envelope-reactive V3-glycan bnAb Lineages in Human and Rhesus Macaque

The paper identifies shared maturation pathways of V3-glycan broadly neutralizing antibody lineages in humans and rhesus macaques, revealing convergent mutations and evolution patterns.

An integrated workflow for structural virology with a 100 keV electron microscope

This paper describes a user-managed 100 keV electron microscope setup for structural virology, enabling high-quality cryo-EM data collection and analysis within a laboratory setting.

Consistent Induction of Broadly Neutralizing HIV Antibodies by a Novel Two-Step Mechanism Informs Immunogen Design

This study shows a novel two-step mechanism for inducing broadly neutralizing HIV antibodies in macaques using a modified SHIV, informing vaccine design.

Env-antibody coevolution identifies B cell priming as the principal bottleneck to HIV-1 V2 apex broadly neutralizing antibody development

The paper identifies B cell priming as the main obstacle to developing HIV-1 V2 apex broadly neutralizing antibodies in SHIV-infected macaques.

Neutralizing antibodies elicited in sequentially immunized macaques recognize V3 residues on altered conformations of HIV-1 Env trimer

This study shows that sequential immunization of macaques with engineered and native-like HIV-1 Env trimers elicits antibodies that neutralize by targeting non-closed Env conformations.