David Arthur Hume

This study uses genetic biosensors to visualize calcium signaling dynamics in macrophages, revealing mechanosensitivity and the effects of Piezo1 channel ablation.

This study shows that CSF1R regulates monocyte differentiation and metabolism, with its blockade reducing Ly6Clow monocytes and affecting subset diversity through the hexosamine biosynthetic pathway.

This study shows that mice with a germ-line deletion of a Csf1r enhancer lack specific macrophages but maintain normal physiology and can be replenished by donor cells.

This study identifies CSF1R-dependent macrophage subpopulations in chickens and shows their critical role in B cell development and function.

This study shows that CSF1R-dependent macrophages are essential for hematopoiesis but many functions of tissue resident macrophages are redundant or strain-specific in mice.

This study identifies a conserved regulatory element in the mouse Csf1r locus that contributes to transcription in hematopoietic and trophoblast cells, with partial redundancy with other enhancers.

This study shows that microglia are not essential for normal postnatal brain development and cognitive function in rats, as Fireko rats lack microglia but show no behavioral differences.

This study investigates the role of IL10 in avian mucosal immunity by generating IL10-deficient chickens, showing altered microbiome composition and enhanced pathogen clearance with increased inflammation.

The paper identifies transcriptomic signatures in aphthous ulcers of Crohn's disease, suggesting bacterial invasion initiates inflammation rather than dysbiosis.

This paper tests whether perisurgical CSF1 treatment reduces liver I/R injury in rats by enhancing macrophage activity and liver regeneration.

This study examines the effects of a loss-of-function mutation in the rat Il34 gene on macrophage development, homeostasis, and inflammation in both the brain and periphery.

This study shows that wild-type bone marrow cells can restore macrophage populations and reverse developmental defects in Csf1rko rats, but not in the brain.

The paper shows that wild-type bone marrow-derived microglia-like cells reduce strain-dependent neuropathology in Csf1r mutant mice by preventing thalamic calcification.

This study shows that neurodevelopmental processes in the cortex and thalamus can occur normally without microglia, using a genetically modified mouse model.

This study examines the impact of a heterozygous Csf1r mutation on brain development and neuropathology in mice, showing reduced microglial abundance and altered gene expression in disease models.

This study shows microglia promote beta-amyloid plaque formation but are not required for subsequent pathological changes in Alzheimer's disease.

This study identifies rare genetic variants associated with extreme DNA methylation levels and shows their impact on gene expression in healthy ageing populations.

This study examines transcriptomic variation in macrophages from IBD families and finds extreme individual variation in gene expression linked to HLA genotype and genetic susceptibility to IBD.

This study shows that complete microglia deficiency accelerates CNS prion disease without increasing prion accumulation, suggesting microglia protect against astrocyte-driven pathology rather than clearing prions.

The paper generates a rat RNA-seq transcriptomic atlas and uses gene correlation networks to identify tissue-specific transcript clusters and biological processes.

This study investigates a kinase-dead Csf1r mutation in mice, showing it causes dominant-negative effects on CSF1R signaling and impacts macrophage development and brain microglia.

This study shows that Csf1r mutation in mice, rats, and humans causes developmental defects, and bone marrow transfer can reverse some phenotypes by restoring macrophages.

Graphia is an open-source platform for graph-based analysis of complex biomedical data, offering visualization, transformation, and analysis tools for high-dimensional datasets.

The paper presents improved pig reference genomes using long-read sequencing, enhancing continuity and accuracy for pig genetics and genomics research.

This study investigates gene expression in sheep cardiovascular tissues to understand vascular calcification, highlighting gene balance and extracellular matrix roles.

This study identifies genetic loci and gene expression patterns associated with Campylobacter resistance in broiler chickens, providing insights for breeding programs.

The paper reports a CRISPR-Cas9-induced deletion and reinsertion event in the mouse Csf1r promoter, highlighting the need for copy number analysis to confirm genomic edits.

The paper assesses the feasibility of improving milk yield and mastitis resistance in dairy sheep through genomic analysis, finding no significant genetic correlation between the traits.

The paper analyzes whole genome sequencing data from an isolated Shetland population, finding increased ultra-rare variants in exonic and regulatory regions compared to mainland Scottish populations.

This study maps the distribution of macrophages in mouse mammary glands across developmental stages using 3D imaging, revealing stage-specific variations and sexual dimorphism.