Gaëlle Legube

Single-cell resolution of resection-dependent chromatin accessibility in response to DNA double-strand breaks reveals specific gene expression programs

The paper examines chromatin accessibility changes at DNA double-strand breaks (DSBs) in single cells, revealing resection-dependent remodeling and associated gene expression programs.

Circadian PERIOD proteins regulate TC-DSB repair through anchoring to the nuclear envelope

The paper identifies PERIOD proteins as regulators of TC-DSB repair by anchoring DSBs to the nuclear envelope, linking circadian rhythms to DNA repair mechanisms.

A novel class of non-coding variants driving DNA double-strand breaks is associated with complex genetic diseases

The paper identifies a new class of non-coding variants, dsbSNPs, that modulate DNA double-strand break frequency and are enriched in GWAS associations for complex diseases.

Endogenous neuronal DNA double-strand breaks are not sufficient to drive brain aging and neurodegeneration

This study shows that neuronal DNA double-strand breaks, even when accumulated due to loss of C-NHEJ, do not drive brain aging or neurodegeneration in mice.

Genome-wide profiling of DNA repair proteins identifies higher-order coordination in single cells

This study identifies higher-order coordination of DNA repair proteins in single cells by analyzing their genome-wide localization using DamID and ChIC sequencing.

ATM-dependent formation of a novel chromatin compartment regulates the Response to DNA Double Strand Breaks and the biogenesis of translocations

The paper identifies a novel chromatin compartment formed by ATM-dependent clustering of damaged TADs, which enhances DNA damage response but increases translocations.

Loop extrusion as a mechanism for DNA Double-Strand Breaks repair foci formation

The paper identifies TADs as functional units in DDR, showing cohesin-mediated loop extrusion contributes to {gamma}H2AX/53BP1 domain formation during DSB repair.

BLM-dependent Break-Induced Replication handles DSBs in transcribed chromatin upon impaired RNA:DNA hybrids dissolution.

This study shows BLM promotes Homologous Recombination at DSBs in active chromatin but switches to Break-Induced Replication when R-loop dissolution is impaired, reducing cell viability.

Predicting double-strand DNA breaks using epigenome marks or DNA at kilobase resolution

The paper predicts double-strand breaks (DSBs) using epigenomic marks and chromatin context, achieving high accuracy without experimental DSB data.