Anass Chiki

Dissecting the differential role of C-terminal truncations in the regulation of aSyn pathology formation and the biogenesis of Lewy bodies

This study investigates how C-terminal truncations of alpha-synuclein (aSyn) affect Lewy body (LB) formation and pathology in Parkinson's disease by analyzing their role in fibril processing and aggregation.

Post-fibrillization nitration of alpha-synuclein abolishes its seeding activity and pathology formation in primary neurons and in vivo

This study shows that nitration of alpha-synuclein after fibrillization reduces its seeding activity and pathology formation in neurons and in vivo.

Neighbouring modifications interfere with the detection of phosphorylated alpha-synuclein at Serine 129: Revisiting the specificity of pS129 antibodies

The paper shows that neighboring post-translational modifications affect the detection of phosphorylated alpha-synuclein at Serine 129 by existing antibodies.

The structural basis of huntingtin (Htt) fibril polymorphism, revealed by cryo-EM of exon 1 Htt fibrils

This study uses cryo-EM to reveal the structural polymorphism of huntingtin exon1 fibrils, showing unique beta-hairpin arrangements and flanking domain organization.

The Nt17 domain and its helical conformation regulate the aggregation, cellular properties and neurotoxicity of mutant huntingtin exon 1

This study shows that the Nt17 domain regulates Httex1 aggregation, toxicity, and cellular uptake through its conformation and interaction with polyQ repeats.

Unraveling the Complexity of Amyloid Polymorphism Using Gold Nanoparticles and Cryo-EM

This paper introduces gold nanoparticles labeled with 11-mercapto-1-undecanesulfonate to visualize and characterize amyloid polymorphism using cryo-EM, revealing structural differences between in vivo and in vitro amyloid fibrils.

A simple, versatile and robust centrifugation-based filtration protocol for the isolation and quantification of α-synuclein monomers, oligomers and fibrils: towards improving experimental reproducibility in α-synuclein research

This paper introduces a centrifugation-based filtration protocol to isolate and quantify α-synuclein monomers, oligomers, and fibrils to improve experimental reproducibility in Parkinson's research.

TBK1 regulates autophagic clearance of soluble mutant huntingtin and inhibits aggregation/toxicity in different models of Huntington's disease

This study identifies TBK1 as a kinase that phosphorylates mutant huntingtin, reduces its aggregation and toxicity in multiple HD models.

The making of a Lewy body: the role of alpha-synuclein post-fibrillization modifications in regulating the formation and the maturation of pathological inclusions.

Calpain-1/2 cleavage of the alpha-synuclein C-terminus after fibril formation is reported to drive seeding, aggregation and the maturation of Lewy-body-like inclusions in primary neurons.

Chronic corticosterone enhancement aggravates alpha-synuclein brain spreading pathology and substantia nigra neurodegeneration in mice

This study shows that chronic corticosterone worsens alpha-synuclein pathology and neurodegeneration in a mouse model of Parkinson's disease.

N-terminal phosphorylation of Huntingtin: A molecular switch for regulating Htt aggregation, helical conformation, internalization and nuclear targeting.

This study investigates how phosphorylation at S13 and S16 of Httex1 affects its structure, aggregation, and subcellular localization, revealing a phosphorylation-dependent switch that regulates its function.