Holly L. Birchenough

Interlaboratory benchmarking of differential scanning fluorimetry reveals sources of variability in protein thermal stability parameters

This study benchmarks DSF across labs, showing variability in thermal stability parameters due to instruments, workflows, and fluorescence readouts, with harmonization and centralized analysis improving consistency.

A High Throughput SPR-Based Array for Quantitative Profiling of Glycosaminoglycan Protein Interactions

The paper introduces a high-throughput SPR array to profile GAG-protein interactions, validating it with known proteins and identifying new interactions.

An avidity-driven mechanism of extracellular BMP regulation by Twisted gastrulation

The paper identifies and validates binding epitopes in Twisted gastrulation (Tsg) that mediate interaction with Sog/Chordin, revealing an avidity-driven mechanism for BMP gradient formation.

Heparan sulfate glycosaminoglycans mediate CXCL4 (PF4) transport across the blood-brain barrier and effects on neurogenesis

This study shows CXCL4 enters the brain via GAG-binding to BBB proteoglycans and promotes neurogenesis through GAG-mediated oligomerization.

The structural organisation of pentraxin-3 and its interactions with heavy chains of inter-alpha-inhibitor regulate crosslinking of the hyaluronan matrix

This paper investigates how PTX3's quaternary structure enables HA crosslinking via interactions with HC1, revealing pH-dependent binding and structural flexibility.