Karel Svoboda

The paper shows that task-related neural activity spreads across a cortical network through task-independent synapses, even in untrained neurons.

The paper describes the Neurodata Without Borders (NWB) ecosystem, which enables FAIR neurophysiological data management and analysis across species and modalities.

The paper shows how midbrain and thalamic circuits reorganize motor cortex dynamics to transition from planning to execution of movement.

The paper develops a workflow to accurately localize electrodes in Neuropixels probes across multiple brains using imaging and electrophysiological data.

The paper shows that attractor dynamics in the frontal cortex gate sensory information flow during decision-making, making choices more robust to distractors over time.

The paper introduces Neuropixels 2.0, a high-density probe for long-term brain recordings, and demonstrates its stability across months in multiple labs and species.

This paper introduces a method to accurately estimate spikes and subthreshold waveforms from 1-photon voltage imaging in vivo, even with noise.

The paper introduces EASI-FISH, a method for spatial gene expression profiling in thick brain sections, and applies it to discover novel subregions in the lateral hypothalamus.

The paper uses targeted photostimulation in mice to investigate neural circuits underlying short-term memory, revealing recurrent interactions and modular organization in the anterior-lateral motor cortex.

The paper introduces a high-throughput instrument called GIZMO to screen fluorescent protein mutants under two-photon excitation, enabling efficient evolution of proteins optimized for this excitation method.

The paper compares neuronal population dynamics measured via calcium imaging and electrophysiology, finding discrepancies that are partially resolved by spike inference algorithms and highlighting the value of forward modeling.

The paper describes engineering eFRET voltage indicators with positive-going fluorescence responses by modifying microbial rhodopsins.

The paper investigates how local recurrent circuits in the somatosensory cortex amplify sensory signals through subnetworks with elevated connectivity, showing that targeted ablation of specific neurons disrupts stimulus encoding.

This study uses calcium imaging in mouse motor cortex pyramidal cells to show that dendritic activity during decision-making is compartmentalized by branching and clustered within branches.

The paper introduces a new genetically encoded voltage indicator, Voltron, which improves brightness and photostability for extended in vivo voltage imaging in mice, zebrafish, and fruit flies.

This paper introduces a two-photon imaging technique that achieves high-speed, high-resolution imaging of neural activity in mice at over 1 kHz frame rates.

The paper benchmarks optogenetic silencers in mice to assess their spatial and temporal effects on cortical circuits, finding that photoinhibition extends beyond light spread and can produce paradoxical effects.

The paper investigates how different types of GABAergic interneurons in the barrel cortex respond to sensory input and whisking, revealing their distinct roles in cortical circuit dynamics.

The paper investigates persistent activity in mouse frontal cortex during a delayed response task and finds evidence for discrete attractor dynamics underlying short-term memory for motor planning.

Version 2.0 of the Neurodata Without Borders neurophysiology format centres on the schema, with an accompanying software stack and community aimed at making recordings easier to reuse.

The paper analyzes neural population dynamics in mouse frontal cortex during decision-making to reveal structure predicting behavioral variability.

The paper reconstructs 1,000 projection neurons in the mouse brain, revealing new cell types and organizational principles of long-range connectivity.

The paper identifies two types of motor cortex neurons with distinct roles in movement preparation and execution using single-cell profiling and axonal reconstructions.

The paper identifies shared and distinct transcriptomic cell types in two mouse neocortical regions using scRNA-seq, showing most excitatory types are region-specific while inhibitory types are shared.

The paper presents transgenic mice expressing the red calcium indicator jRGECO1a for in vivo neuronal imaging, showing comparable or better signal-to-noise ratios than viral methods.

Structure-guided mutagenesis of GCaMP6 plus neuron-based screening yields a jGCaMP7 family, each variant tuned to a different regime: single spikes, neurites and neuropil, or population imaging.

The paper compares preparatory activity in the mouse anterior lateral motor cortex across two tasks and finds similar low-dimensional and monotonic features.

This paper introduces a 2-photon mesoscope with subcellular resolution for in vivo imaging across multiple brain areas in mice.

The paper introduces improved red calcium indicators that match the sensitivity of GFP-based indicators, enabling better in vivo neural activity imaging.