Hirohide Saito

Alignment-free discovery of conserved RNA structures in the human genome

This paper introduces RLMHelix, an alignment-free method to discover conserved RNA structures in the human genome, identifying new candidates that correlate with RNA-binding protein interactions.

A cuffed CRISPR guide RNA for microRNA activity-dependent genome editing

This paper introduces a cuffed guide RNA (cgRNA) that enables miRNA activity-dependent genome editing in human and mouse cells.

Targeting integrin αvβ3 by chimeric antigen receptor neural stem cell (CAR-NSC) therapy for stroke

This paper develops CAR-NSC therapy targeting integrin αvβ3 to improve spatial distribution and tissue engagement of transplanted cells in stroke models.

Transient let-7/miR-98 reprograms primed pluripotency to induce naivehuman PSCs

This paper identifies miR-98-5p as a let-7 family microRNA that enhances naive human PSC induction by repressing NR6A1 and activating BMP2/4 signaling, improving resetting efficiency to ~80%.

Split RNA switch: Programmable and precise control of gene expression by ensemble of pre- and post-translational regulation

The paper introduces split RNA switches that enhance gene regulation specificity by integrating multiple RNA switches through protein splicing, improving ON/OFF ratios and enabling multi-input synthetic circuits.

Programmable mammalian translational modulators by CRISPR-associated proteins

This paper develops CaRTRIDGE, a system using CRISPR-associated proteins to control mRNA translation, creating orthogonal switches and synthetic circuits in mammalian cells.

Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications

This paper develops xeno-free iPSC-derived NPCs compatible with GMP for clinical use, showing long-term survival and differentiation into neurons in vivo.

Post-transcriptionally regulated genes are essential for pluripotent stem cell survival

The paper identifies 20 post-transcriptionally regulated genes essential for pluripotent stem cell survival, showing their protein upregulation and nuclear-to-cytoplasmic mRNA localization.

Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires Bicc1-Ccr4 RNA degradation complex

The paper investigates how fluid flow induces asymmetric Dand5 mRNA decay in the mouse embryo through the Bicc1-Ccr4 RNA degradation complex.

Nanoparticle-based local translation reveals mRNA as translation-coupled scaffold with anchoring function

This study uses a nanoparticle-based method to control mRNA localization and shows mRNA acts as a scaffold for nascent protein function during translation.