Michael Stumpe

Proximity labeling at H3K9me3 reveals VRK-1 regulate global chromatin distribution in C. elegans

This study identifies VRK-1 as a regulator of chromatin-nuclear lamina interactions in C. elegans, showing its role in stress-induced chromatin reorganization and survival.

Sample preparation for mass spectrometry-based tissue (phospho)proteomics

The paper describes a protocol for preparing tissue samples for proteome and phosphoproteome analysis using mass spectrometry, including steps for tissue disruption, lipid depletion, and phosphopeptide enrichment.

The transcription factor LSL-1 interacts with the chromatin factors HIM-17, XND-1 and BRA-2 to promote the germline-specific transcriptional repertoire and to safeguard germ cell fate in C. elegans

The paper identifies LSL-1's interaction with chromatin factors to maintain germline transcriptional programs and cell fate in C. elegans.

Autophagy shapes the peptide repertoire of rheumatoid arthritis-associated HLA class II alleles

This study shows autophagy contributes to MHC class II presentation of RA-related peptides in HLA-DRB1*04:01 alleles, with 30% of peptides differing between autophagy sufficient and deficient cells.

M1-linked Ubiquitination by LUBAC Regulates AMPK Activity and the Response to Energetic Stress

This study shows LUBAC activates AMPK and OTULIN restricts it, affecting metabolic responses to energy stress in cells, mice, and flies.

Sanctioning of bacterial cheaters by the host plant in nitrogen-fixing symbiosis between Medicago truncatula and Sinorhizobium meliloti

The paper investigates how Medicago truncatula sanctions Sinorhizobium meliloti cheaters in nitrogen-fixing symbiosis by altering resource exchange and analyzing metabolic and proteomic changes in nodules.

O-mannosylation and protein maturation check-points represent therapeutic opportunities in BRAF fusion protein oncogenesis

The paper identifies O-mannosylation and protein maturation as therapeutic targets in BRAF fusion oncogenesis by showing their necessity for fusion-induced transformation.

The developing retina undergoes mitochondrial remodeling via PINK1/PRKN-dependent mitophagy

The paper identifies two mitophagy peaks during retinal development, one PINK1-independent and one PINK1-dependent, linked to oxidative stress and RGC differentiation.

Multi-omics analysis of keratinocytes reveals dermokine-dependent regulation of cell-cell adhesion via p120

The paper investigates dermokine's role in keratinocyte adhesion through multi-omics analysis, showing that dermokine loss increases p120 phosphorylation and reduces cell-cell adhesion.

A CELL BIOLOGY STUDY REVEALS NEW INSIGHTS INTO THE TRANSPORT MECHANISMS OF OOMYCETE EFFECTORS

This study identifies the RXLR-EER motif as critical for directing oomycete effectors to the haustorial neck for host cell entry.

Targeted proteomics addresses selectivity and complexity of protein degradation by autophagy

This study uses targeted proteomics to analyze protein degradation patterns during autophagy, revealing differential responses of selective autophagy receptors to various stimuli.

Androglobin, a chimeric mammalian globin, is required for male fertility

This study identifies androglobin (Adgb) as essential for male fertility in mice, showing its role in spermatogenesis and flagellum formation through interactions with Sept10.

Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice

This study shows that deleting Per2 in glial cells of mice alters mood-related behaviors, reducing despair and anxiety with changes in neurotransmitter levels.