Richard Paul Harvey

A scalable MNase-seq framework for reproducible nucleosome profiling across pluripotent stem cell and cardiomyocyte models

The paper develops a scalable MNase-seq workflow for reproducible nucleosome profiling across multiple cell types and models.

Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia

The paper develops a workflow for isolating and profiling adult teleost cardiac cells, revealing exercise-induced hypertrophy and hyperplasia and defining proteo-transcriptomic regulatory networks.

OpenEMMU: a versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics

OpenEMMU improves EdU staining efficiency, brightness, and multiplexing for DNA replication and cell cycle analysis using affordable, open-source reagents.

NKX2-5 congenital heart disease mutations show diverse loss and gain of epigenomic, biochemical and chromatin search functions underpinning pathogenicity

The paper investigates NKX2-5 mutations in congenital heart disease by analyzing their epigenomic, biochemical, and chromatin search functions in cardiomyocytes, revealing diverse loss and gain-of-function defects.

Quantitative trait and transcriptome analysis of genetic complexity underpinning cardiac interatrial septation in mice using an advanced intercross line

This study identifies genetic and molecular factors underlying atrial septal defects in mice using QTL mapping and transcriptome analysis, revealing complex networks and non-coding variants involved.

HOPX governs a molecular and physiological switch between cardiomyocyte progenitor and maturation gene programs

This study identifies HOPX as a molecular switch regulating cardiomyocyte progenitor and maturation gene programs, linking it to cardiac function and genetic variation.

Conserved Role of the Large Conductance Calcium-Activated Potassium Channel, KCa1.1, in Sinus Node Function and Arrhythmia Risk

The paper investigates the role of KCa1.1 in sinus node function and arrhythmia risk across species, finding conserved effects of its loss of function.

Quantitation analysis by flow cytometry shows that Wt1 is required for development of the proepicardium and epicardium

The paper uses flow cytometry to analyze the role of Wt1 in epicardial development, finding that Wt1 is essential for formation and maintenance rather than promoting mesenchymal transition.

Sierra: discovery of differential transcript usage from polyA-captured single-cell RNA-seq data

Sierra identifies differential transcript usage from polyA-captured scRNA-seq data, validated against bulk RNA-seq and showing 3UTR shortening in cardiac fibroblasts.

PDGF signaling directs cardiomyocyte movement toward the midline during heart tube assembly

This study identifies PDGF signaling as essential for guiding cardiomyocyte movement toward the midline during heart tube assembly in zebrafish and mouse.