Zhaofa Wu

This paper introduces Hi-APEX, a H2O2-independent APEX2 variant for in vivo spatial multi-omics, showing improved performance in capturing redox-sensitive processes and enabling in vivo applications.

The paper develops selective fluorescent sensors to detect endocannabinoid dynamics in vivo and shows that Δ9-THC induces sustained 2-AG signaling in the nucleus accumbens.

The paper identifies a female-specific subcortical circuit involving CB1R that maintains defensive homeostasis during risk assessment by modulating GABA release in the lateral habenula.

This paper introduces SeeID, a near-infrared light-controlled proximity labeling method for studying dynamic protein interactomes in vitro and in vivo, revealing proteasome association with Parkin during mitophagy.

This study identifies a stress-induced positive feedback loop in the LHb-LC axis mediated by astrocytic calcium signaling, which influences depressive-like behaviors in mice.

This paper introduces TyroID, an in vivo-compatible method for high-resolution mapping of extracellular proteomes, validated through applications in tumor xenografts and live mouse brains.

This study identifies CALHM2 as an ATP-regulated mitochondrial channel, showing it affects ATP levels and mTOR pathway activity, with implications for aging and disease.

The paper identifies Inflares, spatiotemporally selective ATP events from astrocytes, which encode injury information and guide microglial response, showing their role in both injury amplification and therapeutic reversal.

The paper identifies UDP-P2Y6 signaling as a key driver of microglial calcium activity, phagocytosis, and neuroinflammation during epileptogenesis.

The paper shows astrocytes release ATP spontaneously and non-vesicularly, affecting hundreds of synapses without requiring calcium.

The paper develops a genetically encoded adenosine sensor to show activity-dependent non-vesicular adenosine release from somatodendritic regions via calcium influx and equilibrative transporters.

The paper shows that dopamine activates PFC astrocytes via α1-adrenergic receptors, leading to calcium signals and regulation of extracellular ATP.

This paper introduces a neuropsin-based optogenetic tool, cOpn5, that enables precise control of Gq signaling with high temporal and spatial resolution.

The paper investigates how microglia change their phenotype in acute brain slices and how these changes affect neuronal network activity.

This study investigates how adenosine A1 receptors inhibit dopamine release in the nucleus accumbens, showing that astrocytic ENT1 regulates this inhibition and that ethanol disrupts it.

The paper develops an ultrasensitive GRABATP1.0 sensor to detect extracellular ATP in real time, demonstrating its use in vitro and in vivo across multiple conditions.

The paper identifies SLC35D3 as a vesicular transporter for UDP-glucose through localization, proteomics, and metabolite profiling in neurons.

The paper identifies MRGPRX4 as a novel bile acid receptor involved in cholestatic itch, showing its activation by bile acids and its role in itch induction in humans.

The paper develops GRABNE sensors for rapid, specific in vivo detection of norepinephrine, demonstrating their use in various experimental contexts.