Ricardo Almeida

Ultra-large targeted DNA integrations in primary human cells

This paper identifies methods to achieve ultra-large DNA integrations (>10 kb) in human cells with high efficiency using optimized templates and delivery strategies.

Spike-in probe-enhanced single-cell RNA-seq reveals post-infusion transcriptomic remodeling of "prime-and-kill" synNotch-CAR-T cells

This paper develops a spike-in probe-enhanced scRNA-seq method to track and profile synNotch-CAR-T cells in vivo, revealing tissue-specific activation and transcriptional states.

Synthetic Hybrid Receptors for Safer and Programmable T Cell Therapy

This paper introduces Hybrid Receptors (Hybrid-Rs) to improve T cell therapy safety and programmability by combining CAR and SNIPR features for controlled gene regulation and reduced toxicity.

Programmable microparticles rewire CAR signaling to enable super-physiological expansion of human T cells in vitro

This study uses programmable microparticles to enhance CAR-T cell expansion beyond physiological limits by rewiring signaling pathways.

Three-dimensional connectivity and chromatin environment mediate the activation efficiency of mammalian DNA replication origins

The paper investigates how 3D chromatin structure and chromatin environment influence DNA replication origin activation efficiency in mouse embryonic stem cells under stress and control conditions.