DNA Repair Mechanisms

This study uses FRET assays to show how RPA interactions at P/T junctions facilitate Pol δ holoenzyme assembly and DNA synthesis efficiency.

The paper shows that Mus81 and Yen1 cleave D-loops to form half-crossover precursors in budding yeast.

This study investigates how Rad54 mutations affect homologous recombination outcomes, finding that reduced translocation activity increases genomic rearrangements.

This study reveals how Sae2/CtIP activates Mre11 nuclease activity through structural and biochemical analyses in yeast and human systems.

This study investigates how IHF variants bind and stabilize the open conformation of Holliday junctions.

This paper explains how BRCA2's FxxA and FxPP motifs bind to DMC1 and RAD51 via distinct interfaces, stabilizing recombinase filaments and explaining functional differences between these proteins.

This paper investigates how dysregulated DnaB unwinding in E. coli leads to DNA damage and shows that RecA filamentation helps repair daughter strand gaps and prevent DNA breaks.

This study uses cryo-EM to reveal how XPD helicase stalls on interstrand crosslinked DNA, identifies functional residues, and suggests a model for NER bubble formation.

This study shows that MutL suppresses error-prone DNA mismatch repair and protects noncoding DNA from mutations in yeast and human cells.

The paper identifies PCNA as a substrate of BRCA1/BARD1 ubiquitin E3 activity, showing it promotes replication fork stability and continuous DNA synthesis without requiring Homologous Recomberation or Olaparib resistance.

The paper shows that RAD51 DNA binding site II is essential for recombinogenic activity in Arabidopsis but not for nucleofilament formation.

This study shows that DprA and RecFOR suppress microindel mutations in Acinetobacter baylyi by inhibiting SPDIR, a rare mutation mechanism, and reveals DprA's antimutational role independent of its transformation function.

The paper determines the structure of the LexA-RecA* complex, revealing a lock-and-key mechanism that triggers SOS response gene induction by enabling LexA cleavage.

The paper analyzes mtDNA damage fragments from three species after UV exposure, finding characteristic length profiles and di-pyrimidine patterns suggesting novel repair mechanisms.

This study uses atomic force microscopy to visualize APE1-DNA interactions, revealing APE1's affinity for G-rich DNA and its ability to form loops between DNA segments.

This study shows DBF4, not DRF1, is essential for CDC7 kinase function at replication forks, while both subunits support bulk DNA replication.

This study shows that NucS in mycobacteria is involved in a short-patch DNA mismatch repair mechanism, distinct from canonical MMR, by directly repairing mismatches within 5-6 bp.

The paper investigates mutation mechanisms during homologous recombination in yeast, showing that mismatch repair suppresses quasipalindrome and frameshift mutations during double-strand break repair.

The paper shows genomic uracil induces DNA replication stress without BER processing and identifies UNG2 upregulation as a vulnerability in cancer cells.

This study investigates how HROB regulates the DNA unwinding activity of the MCM8-9 helicase complex through structural and biochemical analyses.

The paper investigates DNA break dynamics at recurrent DNA break clusters (RDCs) in mouse neural progenitor cells, revealing how replication-transcription collisions influence DSB formation and orientation.

The paper identifies POLQ gene mutations in melanoma tumors and shows they reduce DNA polymerase activity and accuracy, potentially contributing to cancer progression.

This study shows RPA is essential for maintaining the meiotic recombination checkpoint in yeast by sensing ssDNA and activating Mek1 kinase to regulate pachytene exit.

This study identifies a physical interaction between Dmc1 and Mlh1-Mlh3 in yeast meiosis and shows that Dmc1 promotes crossover resolution by recruiting Mlh1-Mlh3.

The paper identifies genomic elements and protein interactions that organize meiotic chromosomes for recombination.

This study shows Ki-67 is necessary for DNA replication, genome stability, and prevents DNA damage and interferon response when depleted.

This study shows that specific dimer interactions and ATPase activity in both subunits are required for helicase activity in E. coli Rep and UvrD, with subunit communication demonstrated through Trp fluorescence.

The paper characterizes MrfB, a DEDDh exonuclease involved in repairing mitomycin C-induced DNA damage in Bacillus subtilis, revealing its structural and biochemical properties.

This study investigates how human DNA polymerase β processes the mutagenic lesion Fapy*dG during DNA replication, showing inefficient bypass and incorporation, suggesting mutagenicity arises from bypass rather than lesion incorporation.

The paper shows that microsatellites generate highly mutagenized extrachromosomal circular DNAs through replication-dependent mechanisms and template switching.