Antibiotic Resistance in Bacteria

This study shows that Klebsiella pneumoniae becomes more resistant to beta-lactam antibiotics at low pH due to the activity of specific penicillin-binding proteins.

This study evolves a colistin-resistant Pseudomonas aeruginosa strain using adaptive laboratory evolution, identifies a novel missense mutation in PmrB, and reports fitness trade-offs and collateral sensitivity.

The paper analyzes macrolide resistance mechanisms in bacterial genomes to inform antimicrobial stewardship in resource-limited settings.

The paper shows that glucose availability increases fosfomycin resistance in Enterobacter cloacae by altering GlpT expression and enhancing fosA gene activity.

This study develops Hi-C+ to detect rare plasmid hosts in soil, achieving a 0.001% detection limit and genus-level identification.

The paper tests sulbactam-durlobactam susceptibility in cefiderocol heteroresistant Acinetobacter isolates and finds no heteroresistance, supporting its efficacy and potential prioritization over cefiderocol.

The paper introduces the Sublethal Interaction Factor (SIF) assay to analyze antibiotic combinations at sublethal concentrations, showing high sensitivity and specificity compared to existing methods.

The paper shows that plasmids with antibiotic resistance genes are highly mobile, have broad host ranges, and evolve faster than other plasmids, facilitating the spread of antibiotic resistance across bacterial species.

CARPDM enables cost-effective antibiotic resistome profiling in metagenomic samples through targeted enrichment and in-house probe synthesis.

This paper describes the discovery of TXA11114, an efflux pump inhibitor that enhances levofloxacin efficacy against multidrug-resistant Pseudomonas aeruginosa in vivo with a safe toxicology profile.

This study identifies C7-substituted quinolines as potent inhibitors of AdeG efflux pumps in Acinetobacter baumannii, showing significant antibiotic potentiation and selectivity.

This study identifies and characterizes co-existing blaNDM-1 and blaNDM-5 variants in E. coli isolates using Sanger sequencing and hybrid assembly, and develops a restriction digestion method to distinguish them.

This study uses an Atlantic salmon gut model to show that antimicrobial resistance plasmids persist and transfer among microbes even after antibiotic treatment stops.

This study shows Enterobacter species can survive in human macrophages without replicating and with minimal cytotoxicity.

This study examines genetic and phenotypic changes in Acinetobacter baumannii during a five-year UTI without antibiotic treatment, revealing genome reduction and loss of adhesion capacity but conserved colistin resistance.

The paper identifies a compound that disrupts the TolC-AcrA interface of the E. coli efflux pump, leading to pump disassembly and reduced antibiotic resistance.

This study analyzes the genetic contexts of quinolone resistance genes in environmental E. coli using comparative genomics to identify mobile genetic elements and their chromosomal and plasmid locations.

The paper introduces ROCker models for detecting and typing specific antibiotic resistance genes in short-read sequences, showing improved accuracy over existing methods.

The paper identifies mutations in riboflavin biosynthesis genes that confer resistance to furazolidone and reduce synergy with vancomycin in E. coli.

The paper identifies mutations in Pseudomonas aeruginosa that confer cross-resistance to multiple beta-lactam antibiotics during treatment with ceftazidime/avibactam.

This study evaluates the effectiveness of cefepime-taniborbactam and ceftibuten-ledaborbactam against KPC variants that confer resistance to ceftazidime-avibactam.

The paper identifies three substrate classes for the MexXY-OprM efflux system in Pseudomonas aeruginosa and classifies trimethoprim as a new substrate.

This study investigates de novo antibiotic resistance acquisition in six bacterial species, finding diverse mutational patterns leading to similar resistance outcomes.

This study quantifies how antibiotic use and genetic resistance determinants affect bacterial lineage dynamics using a Bayesian model and Neisseria gonorrhoeae isolates.

This study identifies AMR E. coli in agricultural grasslands and manure, revealing genomic adaptations and resistance mechanisms.

The paper estimates within-host mutation rates of ESBL-producing E. coli and K. pneumoniae using phylodynamic models on longitudinal genomic data from hospitalized patients.

This study compares plasmid datasets to assess how data origin affects inferences about plasmid mobility, antimicrobial resistance, virulence, and host taxonomy.

This study compares antimicrobial resistance profiles in bacterial isolates from stable flies and bovine manure, identifying resistance genes and plasmids associated with clinically relevant taxa.

This study develops a neonatal mouse model to investigate gut colonization by carbapenem-resistant Enterobacter hormaechei, revealing mucus metabolism as a key factor in intestinal colonization.

This study analyzes AMR in urban microbiomes using metagenomic data, finding that AMR++ and Bowtie are effective for detecting AMR genes and that MGEs significantly impact resistome modeling.