Advanced Fluorescence Microscopy Techniques

This study maps condensate dielectric permittivity using fluorescence and reveals that membrane interactions depend on permittivity contrast rather than absolute permittivity.

This paper introduces a symmetrically dispersed dual-wedge prism design for spectroscopic single-molecule localization microscopy, improving lateral and spectral precision.

This paper introduces Atacama Clear (ATC), a 3D imaging technology that improves resolution and signal detection in cleared tissues, enabling better analysis of complex biological samples.

This paper introduces a dual-color miniature endoscope for simultaneous calcium imaging of two neuronal populations in behaving mice, revealing direction-dependent activity patterns in striatal neurons.

The paper introduces Caterpie, a live cell labeling system using split fluorescent proteins to enable high-fidelity identification of multiple cell populations with 97% accuracy.

This paper introduces a high-affinity split-HaloTag system for live-cell protein labeling, enabling efficient and versatile imaging with compatibility for advanced microscopy techniques.

This paper introduces a method for nanoscale drift stabilization in thick non-transparent tissues using back-illumination phase imaging and cross-correlation analysis, enabling high-quality imaging of extracellular structures.

This study investigates dark state-mediated photobleaching in mCherry-based RFPs, showing photodestructive dark states and providing insights for engineering brighter and more photostable probes.

The paper introduces MiniFAST, a miniaturized microscope that improves speed, sensitivity, and resolution for in vivo neural recording using genetically encoded indicators.

This paper introduces a wash-free method for multi-target super-resolution microscopy using photocaged DNA labels, demonstrating improved imaging accuracy without sample manipulation.

This paper develops a genetically encoded fluorescent sensor, GRABPGE2-1.0, to monitor spatiotemporal PGE2 dynamics in vivo with high resolution.

This paper develops a minimally invasive optical clearing medium for live mammalian tissue imaging, enabling structural and functional imaging with minimal impact on cellular properties.

The paper introduces a live-cell imaging method combining spectral linear unmixing and ratiometric FRET to quantify protein interactions and signaling dynamics with high spatial and temporal resolution.

FLEXTAG improves super-resolution imaging by enabling long-term, multi-color, low-bleaching labeling of subcellular structures in live and fixed cells.

This study shows fluorescence intensity decreases over six weeks in IHC, influenced by antibody choices and staining methods, highlighting the need for imaging best practices.

This paper investigates how absorption dipole orientation affects MINFLUX localization accuracy, finding that tilted dipoles introduce biases that depend on their orientation and position within the imaging area.

The paper introduces GCaMP8 variants targeted to synaptic compartments and develops CaFire software to quantify Ca2+ signals, showing improved sensitivity and resolution compared to existing methods.

The paper develops an ultrasensitive ratiometric glucose sensor to monitor glucose transport and metabolism in plants, finding rapid hydrolysis of shoot-derived sucrose in root tips.

The paper quantitatively evaluates nine green and eight red fluorophores in Drosophila embryos to identify optimal choices based on bleaching, folding, and imaging conditions.

This paper develops a method to estimate the separation of two freely rotating dipole emitters near the quantum limit using vectorial theory and a modified SLIVER technique.

This study demonstrates that rhodopsin-based voltage indicators can be used for two-photon voltage imaging, achieving high-fidelity action potential detection in vitro and in vivo.

The paper introduces curved axially scanned light-sheet microscopy to overcome field curvature limitations in light-sheet imaging, increasing the usable field of view while maintaining sub-cellular resolution.

This study uses NAD(P)H FLIM to map metabolic profiles in Drosophila mushroom bodies, revealing spatial heterogeneities and learning-induced metabolic changes.

Altair-LSFM is a high-resolution, open-source light-sheet microscope designed for sub-cellular imaging, achieving 235 nm lateral and 350 nm axial resolution.

The paper develops an optimized immunolabeling protocol to quantify endogenous membrane receptor stoichiometry using single-molecule localization microscopy on Jurkat T cells and primary multiple myeloma cells.

This paper develops a phase separation-based PINK1 biosensor to visualize real-time PINK1 activity in single live cells and study its role in mitophagy.

This paper introduces caging-group-free photoactivatable fluorophores with far-red emission for improved super-resolution microscopy.

The paper develops a glucose biosensor, TINGL, for measuring intracellular glucose dynamics in yeast and human cells, showing it detects glucose concentrations up to 1 mM and responds to glucose addition in starved cells.

This paper develops dual-color expansion microscopy using non-canonical amino acid labeling to achieve high-resolution imaging of membrane proteins in HEK 293T cells.

This paper introduces SP-STORM, a method combining single molecule photoswitching and spectral phasor analysis to achieve high-throughput, multiplexed super-resolution imaging of subcellular structures.