Biosensors and Analytical Detection

Novel triplex nucleic acid lateral flow immuno-assay (NALFIA) for rapid detection of Nipah virus, Middle East respiratory syndrome coronavirus and Reston ebolavirus

The paper develops a triplex lateral flow assay for detecting Nipah virus, MERS-CoV, and REBOV genomes with high sensitivity and specificity.

Detection of Frog virus 3 via the system integrating RPA-CRISPR/Cas12a-SPM with deep learning

This paper develops a rapid, sensitive, and point-of-care FV3 detection system combining RPA-CRISPR/Cas12a with smartphone microscopy and deep learning, achieving 100% accuracy in classification.

Electricity-free nucleic acid extraction method from dried blood spots on filter paper for point-of-care diagnostics

The paper develops an electricity-free nucleic acid extraction method from dried blood spots on filter paper for point-of-care diagnostics, showing comparable performance to standard methods.

A comparison between Mini-loop mediated isothermal amplification and polymerase spiral reaction for selective amplification of short template DNA

This study compares AS-Mini-LAMP and PSR for amplifying short DNA templates, finding AS-Mini-LAMP more effective for mutant allele detection.

Quantitative Isothermal Amplification on Paper Membranes using Amplification Nucleation Site Analysis

This paper develops a paper-based isothermal amplification method for rapid nucleic acid quantification using fluorescent nucleation site analysis, achieving results in under 20 minutes.

Rapid visual CRISPR assay: a naked-eye colorimetric detection method for nucleic acids based on CRISPR/Cas12a and convolutional neural network

This paper develops a CRISPR/Cas12a-based colorimetric assay with a convolutional neural network for rapid, naked-eye detection of viral nucleic acids in point-of-care testing.

Point-of-care analyte quantification and digital readout via lysate-based cell-free biosensors interfaced with personal glucose monitors

This paper demonstrates the use of lysate-based cell-free biosensors integrated with personal glucose monitors for on-site analyte quantification.

Protocell Arrays for Simultaneous Detection of Diverse Analytes

This paper introduces a protocell array platform for simultaneous detection of diverse analytes, demonstrating its ability to measure small molecules, minerals, and nucleic acids from a single sample.

An Attempt to Develop an Aptamer Lateral-Flow Assay (ALFA) for Easy, Rapid, and Sensitive Detection of Lethal Mushroom Toxin α-amanitin

This study attempts to develop an aptamer lateral-flow assay (ALFA) for detecting α-amanitin but fails to produce a colorimetric readout due to challenges with small molecule immobilization and binding mechanisms.

Direct multiplex recombinase polymerase amplification for rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa in food

This study develops a direct multiplex RPA method for rapid, sensitive detection of S. aureus and P. aeruginosa in food samples without DNA extraction.

A Chemical-Enhanced System for CRISPR-Based Nucleic Acid Detection

This paper introduces CECRID, a CRISPR-based detection system enhanced with L-proline to improve sensitivity and robustness for nucleic acid detection, particularly for SARS-CoV-2.

DIRECT DETECTION OF LISTERIA MONOCYTOGENES BY RECOMBINASE POLYMERASE AMPLIFICATION

This study develops a rapid, sensitive RPA method for detecting L. monocytogenes in crude samples, achieving 1000-fold higher sensitivity than conventional PCR.

Direct colorimetric LAMP assay for in-field detection of African swine fever virus: a validation study during an outbreak in Vietnam

This study develops and validates a colorimetric LAMP assay for rapid, in-field detection of African swine fever virus using crude serum samples during an outbreak in Vietnam.

A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum

This paper develops a low-cost smart system for DNA/RNA detection using visible light and IoT techniques, enabling qualitative and semi-quantitative analysis without UV equipment.

Rapidemic, a versatile and label-free DNAzyme-based platform for visual nucleic acid detection

Rapidemic is a label-free DNAzyme-based platform for nucleic acid detection using RPA and LSDA, demonstrating visible color change without complex instrumentation.

"Scentsor": A Whole-Cell Yeast Biosensor with an Olfactory Reporter for Low-Cost and Equipment-Free Detection of Pharmaceuticals

This paper introduces a yeast biosensor that detects pharmaceuticals via scent, requiring no equipment, and demonstrates its sensitivity to estradiol in low-resource settings.

RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors

The paper introduces RPA-PCR couple to bypass DNA extraction in plant diagnostics, achieving specific detection of Phytophthora pathogens with sensitivity down to 10 fg of genomic DNA.

Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

This paper develops CRISPR-Cas13a-based diagnostics for real-time detection of Ebola and Lassa viruses in field settings, demonstrating sensitivity and usability in clinical samples.

Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout

This paper introduces Apta-NASBA, a method for highly specific, multiplexed pathogen detection using fluorescent aptamers, achieving a 1 pM detection limit and compatible with various detection platforms.

Development and on-site evaluation of an easy-to-perform and low-cost food pathogen diagnostic workflow for low-resource communities

This paper develops a low-cost food pathogen diagnostic workflow for low-resource settings and demonstrates its effectiveness in detecting E. coli O157 contamination in Cambodian produce.

An isothermal method for sensitive detection of Mycobacterium tuberculosis complexes using CRISPR/Cas12a cis- and trans-cleavage

This paper develops a CRISPR/Cas12a-based method for detecting Mycobacterium tuberculosis with high sensitivity and specificity using RPA and fluorescence detection.

Cas12a-based on-site and rapid nucleic acid detection of African swine fever

This paper develops a rapid, sensitive, and on-site ASFV detection system using RAA-Cas12a and lateral-flow strips, achieving femtomolar sensitivity in an hour.

Nucleic acid amplification by a transparent graphene Visual-PCR chip and a disposable thermocycler

This paper presents a low-cost, portable PCR platform using a graphene-based chip and disposable thermocycler for nucleic acid amplification, enabling visual monitoring and point-of-care testing.

Multiple Cross Displacement Amplification Coupled with Gold Nanoparticles-Based Lateral Flow Biosensor for Detection of the Mobilized Colistin Resistance Gene mcr-1

This paper develops a rapid, sensitive, and specific MCDA-LFB assay for detecting the mcr-1 gene in clinical and livestock samples.

PLANT-Dx: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens

This paper develops a portable, low-cost diagnostic system for detecting plant pathogens using synthetic biology and isothermal amplification with colorimetric output.

Multiplex logic processing isothermal diagnostic assays for an evolving virus

This paper develops a multiplex isothermal diagnostic assay for detecting variable viral targets, demonstrating its effectiveness against divergent Zika virus lineages.

A low-cost biological agglutination assay for medical diagnostic applications

This paper develops a low-cost agglutination assay using E. coli-displayed nanobodies for detecting biomarkers with high selectivity and low detection limits, suitable for point-of-care testing.

Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone

This paper develops a LAMP-OSD assay with cellphone imaging for rapid, accurate detection of mosquito species and Wolbachia infection in field samples.