Digital Holography and Microscopy

Quantitative Phase Imaging of Epithelial Monolayer Dynamics

This study measures height, volume, and mass dynamics of MDCK monolayers using two QPI techniques and develops new methods for cell segmentation and height statistics without image segmentation.

Label-Free Mapping of Subcellular Dynamics using Wide-field Interferometric Scattering Microscopy and Spectral Exponent Analysis

This paper uses wide-field iSCAT microscopy and spectral exponent analysis to label-free map subcellular dynamics, distinguishing between different cellular states and cancer subtypes.

Holotomography-driven learning for in-silico staining of single cells in flow cytometry avoiding co-registration

This paper introduces a Holotomography-driven learning method for virtual staining in flow cytometry without requiring co-registration, enabling label-free single-cell analysis.

Maskless and on-chip LED-array microscope with spatially-varying angle calibration for centimeter-scale phase imaging

This paper presents a computational framework for mask-free on-chip phase imaging with adaptive calibration of spatially-varying illumination angles, achieving centimeter-scale imaging with micron-level resolution.

Label-Free In-Line Characterization of Immune Cell Culture using Quantitative Phase Imaging

This paper introduces qOBM for non-destructive, label-free monitoring of T cell cultures, showing strong correlation with traditional assays and potential for improving cell therapy development.

Microbial Cell-Adaptive Segmentation in Quantitative Phase Imaging

The paper introduces mCAS, a segmentation framework for QPI that outperforms existing methods in accuracy and speed without requiring training data or specialized hardware.

Disordered glass nanowire substrates produce in vivo-like astrocyte morphology revealed by optical diffraction tomography

This study uses optical diffraction tomography to show that disordered glass nanowire substrates induce in vivo-like astrocyte morphology in cultured rat astrocytes.

Quantitative phase deformability cytometry (QP-DC) for precise and clinically relevant multiparametric immune cell profiling

QP-DC combines QPI and deformability cytometry to measure biophysical and mechanical properties of immune cells, revealing LPS-induced changes in neutrophils and heterogeneity in SLE patients.

On-The-Fly Live-Cell Intrinsic Morphological Drug and Genetic Screens by Gigapixel-per-second Spinning Arrayed Disk Imaging

This paper introduces SpAD, a high-throughput label-free imaging platform for live-cell drug and genetic screens, enabling rapid, unbiased profiling of cellular responses.

Comparative Analysis of Stain-Free Fourier Ptychographic Microscopy and Traditional Histopathological Light Microscopy in Renal Membranous Nephropathy

This study compares stain-free Fourier Ptychographic Microscopy (FPM) with traditional histopathological light microscopy in diagnosing membranous glomerulonephritis, finding FPM provides better contrast in glomerular membranes.

Organelle-Aware Representation Learning Enables Label-Free Detection of Mitochondrial Dysfunction in Live Human Neurons

This paper introduces a noninvasive framework using optical diffraction tomography and organelle-aware representation learning to detect mitochondrial dysfunction in live human neurons with high accuracy.

Determination of Drug Sensitivity in Patient Derived Models of Breast Cancer by Multiparametric QPI

This paper demonstrates that multiparametric QPI can assess drug sensitivity in patient-derived breast cancer models, including cryopreserved samples, revealing cellular heterogeneity and resistance mechanisms.

Self-consistent automatic retrieval of single cell rotation enables highly reliable holo-tomographic flow cytometry

This paper introduces a self-consistent method for automatically retrieving single cell rotations in holo-tomographic flow cytometry, achieving full automation and superior accuracy.

Label-Free, Real-Time, In Vivo Optical Biopsy with a Handheld Quantitative Phase Microscope

This paper develops a handheld quantitative phase microscope for real-time, label-free in vivo imaging of tissues with high resolution and low cost.

Total variation of quantitative phase map reveals cellular Young's modulus

This study uses quantitative phase microscopy to measure cellular Young's modulus in real time and finds that total variation of phase correlates with mechanical properties, revealing differences in stiffness between cell types in wound healing.

Multivalency controls the growth and dynamics of a biomolecular condensate

This study uses holographic microscopy to analyze how multivalent ions affect the growth and dynamics of PopZ condensates, revealing deviations from classical models and highlighting the technique's advantages.

Fabry-Perot Microscopy for Improved Contrast Enhancement and 3D Cellular Imaging

This paper introduces Fabry-Perot Microscopy (FPM), a label-free technique that enhances contrast and enables 3D imaging of transparent biological samples with up to 20-fold improvement over conventional wide-field microscopy.

Determination of the absolute concentration of Rayleigh particles via scattering microscopy

This paper introduces iCAUNT, a method to measure the absolute concentration of nanoparticles smaller than one-tenth of the imaging light wavelength using scattering microscopy.

Label-free classification of cell death pathways via holotomography-based deep learning framework

This paper develops a label-free deep learning framework using holotomography to classify cell death pathways with high accuracy.

Cell-TIMP: Cellular Trajectory Inference based on Morphological Parameter

The paper introduces a method using quantitative phase imaging and genomic analysis tools to infer cellular trajectories based on morphology, revealing morphological patterns in leukemia and breast cancer progression.

Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization

This study uses holotomographic microscopy to visualize endothelial cell dynamics during endothelialization, revealing insights into lipid droplet and mitochondrial behavior.

Polarization gratings aided common-path Hilbert holotomography for high-throughput lipid droplets content assay

This paper introduces a novel CP-HHT method for high-throughput 3D refractive index imaging of lipid droplets, showing its ability to distinguish between cholesterol esters and triacylglycerol-rich droplets.

Optical tomography reconstructing 3D motion and structure of multiple-scattering samples under rotational actuation

This paper develops an optical tomography method to reconstruct 3D motion and structure of multiple-scattering samples under rotational actuation, achieving isotropic resolution for biomedical applications.

Using rotational integration of oblique interferometric scattering (RO-iSCAT) to track axial spatiotemporal responses of membrane protrusions

This paper introduces RO-iSCAT, a technique that enables real-time, label-free tracking of membrane protrusions with high axial resolution and minimal phototoxicity.

Label-Free Visualization and Segmentation of Endothelial Cell Mitochondria Using Holotomographic Microscopy and U-Net

The paper develops a U-Net model to segment mitochondrial networks in endothelial cells using holotomographic microscopy images.

HoloMoA: Fast holography and deep-learning-based tool for the novelty detection of mechanism of action of antimicrobial candidates

HoloMoA uses holography and deep learning to classify and detect novel mechanisms of action of antimicrobials with 89% accuracy on known antibiotics.

Single-shot label-free nanoscopy for quantitative organelle visualization on standard commercial microscopes

This paper introduces UpDPC, a label-free microscopy technique that enhances resolution and sensitivity for subcellular organelle visualization on standard microscopes.

Uncovering Astrocyte Morphological Dynamics Using Optical Diffraction Tomography and Shape-based Trajectory Inference

This study uses optical diffraction tomography to analyze astrocyte morphology changes in postnatal rat cortical astrocytes over 7 days in vitro, revealing dynamic shape transitions and cellular composition shifts.

Quadrant darkfield (QDF) for label-free imaging of intracellular puncta

This paper introduces a label-free imaging method called quadrant darkfield (QDF) to enhance subcellular structure analysis in living cells, showing improved resolution and consistency compared to traditional darkfield.

An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization

The paper introduces the Omni-Mesoscope, a cost-effective imaging platform that combines quantitative phase microscopy and multiplex fluorescence imaging to study cellular dynamics and molecular characteristics.