A. N. Engelman

Spatiotemporal binding of cyclophilin A and CPSF6 to capsid regulates HIV-1 nuclear entry and integration

This study shows that sequential binding of CypA and CPSF6 to HIV-1 capsid is necessary for nuclear entry and integration, and that lenacapavir targets this process.

Oligomeric HIV-1 Integrase Structures Reveal Functional Plasticity for Intasome Assembly and RNA Binding

This study determines cryo-EM structures of HIV-1 integrase tetramers and intasome hexadecamers, revealing structural plasticity and functional roles in viral integration and RNA binding.

Multivalent interactions essential for lentiviral integrase function

This study reports cryo-EM structures of the lentiviral intasome and shows that multivalent interactions are essential for integrase function and virus infectivity.

Capsid lattice destabilization leads to premature loss of the viral genome and integrase enzyme during HIV-1 infection

This study shows that destabilizing the HIV-1 capsid lattice leads to premature loss of the viral genome and integrase enzyme in infected cells.

Integrase-RNA interactions underscore the critical role of integrase in HIV-1 virion morphogenesis

This study identifies three mechanisms by which class II HIV-1 integrase substitutions disrupt IN-RNA interactions, leading to virion maturation defects and premature viral genome degradation.