Alan Engelman

HIV-1 Ribonucleoprotein Complex Organization Facilitates Assembly of the Mature HIV-1 Capsid

This study uses molecular dynamics simulations to show that IN-RNA filaments guide HIV-1 capsid assembly and shape, with octameric filaments forming more conical capsids than tetrameric ones.

Development and validation of HIV SMRTcap for the characterization of HIV-1 reservoirs across tissues and subtypes

The paper introduces HIV SMRTcap, a method for characterizing HIV-1 reservoirs by identifying integration sites and proviral integrity using long-read sequencing, and validates its performance across subtypes and tissue samples.

Correlative In Situ Cryo-ET Reveals Cellular and Viral Remodeling Associated with Selective HIV-1 Core Nuclear Import

This study uses cryo-correlative techniques to analyze HIV-1 nuclear import, revealing dependencies on capsid elasticity, nuclear pore adaptability, and CPSF6 binding.

Timed chromatin invasion during mitosis governs prototype foamy virus integration site selection and infectivity

This study shows that PFV Gag protein interacts with mitotic chromatin, and altered binding affects integration site selection and infectivity.

Structural and Mechanistic Bases for Resistance of the M66I Capsid Variant to Lenacapavir

This paper investigates how the M66I mutation in HIV-1 capsid protein confers resistance to lenacapavir by altering inhibitor binding and viral replication.

CPSF6 Promotes HIV-1 Preintegration Complex Function

This study shows CPSF6 promotes HIV-1 preintegration complex function by enhancing viral DNA integration into gene-dense regions and restoring integration activity when added back to mutant cells.

Spatiotemporal binding of cyclophilin A and CPSF6 to capsid regulates HIV-1 nuclear entry and integration

This study shows that sequential binding of CypA and CPSF6 to HIV-1 capsid is necessary for nuclear entry and integration, and that lenacapavir targets this process.

The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir

This study identifies the mechanism by which lenacapavir inhibits HIV-1 maturation by altering capsid protein assembly.

Interplay between the cyclophilin homology domain of RANBP2 and MX2 regulates HIV-1 capsid dependencies on nucleoporins

This study shows that the RANBP2-Cyp domain influences HIV-1 infection and MX2 activity by modulating CA interactions with nucleoporins and integration targeting.

The nuclear localization signal of CPSF6 governs post-nuclear import steps of HIV-1 infection

This study investigates how the nuclear localization signal (NLS) of CPSF6 influences HIV-1 infection after nuclear import, showing that some NLSs impair viral integration.

Disruption of LEDGF/p75-directed integration derepresses antisensetranscription of the HIV-1 genome

The paper shows that disrupting HIV-1 integrase interactions with LEDGF/p75 increases antisense RNA (asRNA) production in HIV-1 infected cells.

The role of LEDGF in transcription is exploited by HIV-1 to position integration

This study shows LEDGF is recruited to promoters by MLL1 and recruits RNA Pol II, facilitating HIV-1 integration across gene bodies.