P. K. Singh

New paradigm of CRISPR spacerome for classification of global Escherichia coli lineages

The paper identifies a conserved CRISPR spacerome signature across globally distributed E. coli strains, suggesting its potential as a marker for epidemiological tracking.

Spatiotemporal binding of cyclophilin A and CPSF6 to capsid regulates HIV-1 nuclear entry and integration

This study shows that sequential binding of CypA and CPSF6 to HIV-1 capsid is necessary for nuclear entry and integration, and that lenacapavir targets this process.

Lecanemab Blocks the Effects of the Aβ/Fibrinogen Complex on Blood Clots and Synapse Toxicity in Organotypic Culture

This study shows lecanemab blocks Aβ/fibrinogen complex formation, reducing clot abnormalities and synaptotoxicity in AD models.

SPACe (Swift Phenotypic Analysis of Cells): an open-source, single cell analysis of Cell Painting data

SPACe is an open-source tool for analyzing Cell Painting data, offering faster processing and better handling of cell-to-cell variation compared to existing software.

Identifying the genetic and epigenetic basis for asymmetric bZIP expression in temperature-stressed bread wheat

This study investigates the genetic and epigenetic factors underlying asymmetric bZIP expression in bread wheat under temperature stress.

Mitochondrial DNA released by senescent cells triggers immunosuppression in cancer

This study shows that mtDNA released by senescent cells triggers immunosuppression via PMN-MDSCs, and inhibiting this pathway enhances therapy-induced senescence in cancer.

Targeting the glucose-insulin link in head and neck squamous cell carcinoma induces cytotoxic oxidative stress and inhibits cancer growth

This study shows targeting GLUT1 and inducing oxidative stress in HNSCC inhibits cancer growth and identifies insulin's role in modulating HNSCC metabolism.

Heterologous Expression and Characterization of ToxA1 haplotype from India and its interaction with Tsn1 for Spot Blotch Susceptibility in spring wheat

This study clones and expresses the ToxA1 haplotype from B. sorokiniana, showing its interaction with Tsn1 in wheat to determine susceptibility to spot blotch.

DHODH inhibition enhances the efficacy of immune checkpoint blockade by increasing cancer cell antigen presentation

This study shows DHODH inhibition enhances immune checkpoint blockade by increasing cancer cell antigen presentation through pyrimidine depletion and P-TEFb-mediated RNA polymerase II elongation.

Integrated single-cell analysis defines the epigenetic basis of castration-resistant prostate luminal cells

The paper uses multi-omics single-cell data to define the epigenetic basis of castration-resistant prostate luminal cells in mice and identifies key molecular pathways involved.

Host selection has stronger impact on leaf microbiome assembly compared to land-management practices

This study shows host selection has a stronger impact on leaf microbiome assembly than land-management practices in Eucalyptus saligna plantations.

Self-inhibition of HER2 at fever temperatures may prevent dimerization with HER3

The paper uses molecular dynamics simulations to show that HER2 adopts an inactive conformation at 40°C, preventing dimerization with HER3, suggesting fever temperatures could modulate HER2 activity in cancer treatment.

Coenzyme A governs proinflammatory macrophage metabolism

This study shows that exogenous CoA enhances TLR-driven proinflammatory and antimicrobial responses in macrophages by promoting oxidative metabolism and histone acetylation.

Targeting keratin 17-mediated reprogramming of de novo pyrimidine biosynthesis to overcome chemoresistance in pancreatic cancer

This study identifies K17-mediated pyrimidine biosynthesis as a mechanism of chemoresistance in PDAC and shows that targeting DHODH with Brequinar enhances the efficacy of chemotherapy.

Cryo-EM structure of the Type IV pilus extension ATPase from enteropathogenic Escherichia coli

The paper reports the cryo-EM structures of the BfpD ATPase from EPEC in different nucleotide states, revealing divergent quaternary configurations and suggesting a new mechanism for T4P extension.

A pandemic clonal lineage of the wheat blast fungus

The paper identifies a pandemic clonal lineage of the wheat blast fungus that originated in South America and spread to Asia and Africa, showing it can be controlled by Rmg8 but may develop fungicide resistance and recombine with African lineages.

Multivalent interactions essential for lentiviral integrase function

This study reports cryo-EM structures of the lentiviral intasome and shows that multivalent interactions are essential for integrase function and virus infectivity.

Eicosanoids in the pancreatic tumor microenvironment: a multicellular, multifaceted progression

The paper identifies key eicosanoid changes in pancreatic tumorigenesis and the cell types responsible for their synthesis using mouse models and patient samples.

The Actin Bundling Protein Fascin Promotes Lung Cancer Growth and Metastasis by Enhancing Glycolysis and PFKFB3 Expression

This study shows that fascin promotes lung cancer growth and metastasis by enhancing glycolysis through YAP1-PFKFB3 activation.

Chronological lifespan extension and nucleotide salvage inhibition in yeast by isonicotinamide supplementation

This study shows that isonicotinamide (INAM) extends yeast chronological lifespan by inhibiting nucleotide salvage pathways, leading to NAD+ accumulation and a hormetic stress response.

A Population-level Strain Genotyping Method to Study Pathogen Strain Dynamics in Human Infections

This paper introduces PopMLST, a method to detect strain-level changes in bacterial infections by analyzing PCR-amplified polymorphic loci and next-generation sequencing data.

The depletion mechanism can actuate bacterial aggregation by self-produced exopolysaccharides and determine species distribution and composition in bacterial aggregates

This study shows depletion aggregation driven by P. aeruginosa exopolysaccharides influences bacterial species distribution and composition in mixed cultures.

Vibrio cholerae biofilm dispersal regulator causes cell release from matrix through type IV pilus retraction

This study identifies VbdR as a regulator of Vibrio cholerae biofilm dispersal, showing it induces type IV pilus retraction and matrix protease expression to release cells from the biofilm matrix.

Contrasting processing tomato cultivars unlink yield and pollen viability under heat stress

This study compares two processing tomato cultivars under heat stress, finding that yield differences are not linked to pollen viability, suggesting other factors are key to heat tolerance.

Temporal resolution of melanogenesis determine fatty acid metabolism as key skin pigment regulator

The paper identifies fatty acid metabolism as a key regulator of melanogenesis by analyzing temporal metabolic and transcriptional changes in melanocytes.

Indoleamine 2,3-dioxygenase upregulates PD-1 expression on ovarian tumor infiltrating CD8+ T cells via kynurenine activation of the aryl hydrocarbon receptor

This study shows that IDO1 promotes PD-1 expression on CD8+ T cells in ovarian tumors through kynurenine-mediated AHR activation, suggesting therapeutic targeting of these pathways.

Matrix-trapped viruses can protect bacterial biofilms from invasion by colonizing cells

The paper shows that phages trapped in biofilm matrices can kill incoming bacteria, but prolonged exposure allows incoming cells to become protected by envelopment in curli clusters.

Reduced NCOR2 expression accelerates androgen deprivation therapy failure in prostate cancer

This study shows reduced NCOR2 expression accelerates androgen deprivation therapy failure in prostate cancer by promoting DNA hypermethylation and neuroendocrine gene expression.

Hypoxia driven oncometabolite L-2HG maintains "stemness"-differentiation balance and facilitates immune suppression in pancreatic cancer

This study shows hypoxia drives L-2HG production in pancreatic tumors, maintaining stemness-differentiation balance and promoting immune suppression, with LDH inhibition reversing these effects.

Tuft cells restrain pancreatic tumorigenesis through paracrine eicosanoid signaling

This study shows that tuft cells suppress pancreatic tumorigenesis by producing anti-inflammatory prostaglandin D2, which inhibits stromal activation, and identifies their protective role in early disease progression.