Etsuko Shibata

Metazoan Orc6 Proteins Evolved Alternative Mechanisms for Association with the ORC Complex: Insights from Drosophila Modeling

The paper investigates differences in Orc6 protein interactions with the ORC complex in Drosophila and humans, proposing that a nuclear localization signal in humans compensates for weaker binding.

Chromatin regulator HELLS mediates SSB repair and responses to DNA alkylation damage.

This study identifies HELLS as a regulator of SSB repair and DNA alkylation damage response, showing its synthetic lethality with HRD in cancer.

Regulation of epigenetics and chromosome structure by human ORC2

This study investigates the role of ORC2 in epigenetic regulation and chromosome structure through a multi-omics analysis of a human cell line with ORC mutations.

Specific origin selection and excess functional MCM2-7 loading in ORC-deficient cells.

The paper shows that specific DNA replication origins are still used and excess MCM2-7 is loaded in ORC-deficient human cancer cells.

DNA replication in primary hepatocytes without the six-subunit ORC

This study shows that primary hepatocytes can replicate DNA without ORC2, similar to cancer cell lines, by loading MCM2-7 complexes.

Integrative analysis of DNA replication origins and ORC/MCM binding sites in human cells reveals a lack of overlap

The paper analyzes DNA replication origins and ORC/MCM binding sites in human cells, finding minimal overlap and suggesting origins are specified by stochastic events rather than specific binding sites.

A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins

The paper shows that human cancer cells can initiate DNA replication without ORC5 and ORC2 proteins, suggesting alternative mechanisms for MCM2-7 recruitment.

ATAC-seq identifies thousands of extrachromosomal circular DNA in cancers and cell lines

The paper shows that ATAC-seq can detect thousands of extrachromosomal circular DNAs in cancer and cell lines without specific enrichment.

The role of ANGIOGENIN, or lack thereof, in the generation of stress-induced tRNA halves and of smaller tRNA fragments that enter Argonaute complexes

This study shows ANG cleaves specific tRNAs to produce halves and tRF-5s, but stress-induced tRNA halves are mostly ANG-independent, and tRF-3s are not generated from tRNA halves or ANG cleavage.