T. Soldati

Discovery of anti-infective compounds against Mycobacterium marinum after biotransformation of simple natural stilbene scaffolds by a fungal secretome

This study identifies trans-δ-viniferins as promising anti-infective scaffolds against Mycobacterium marinum using a Dictyostelium discoideum infection model and natural product derivatives.

An evolutionary-conserved VPS34-PIKfyve-TRPML1-Myosin II axis regulates the speed of amoeboid cell migration

This study identifies a conserved VPS34-PIKfyve-TRPML1-Myosin II axis that controls the speed of amoeboid cell migration by regulating retrograde actin flow through Ca2+ efflux.

Temporal genome-wide fitness analysis of Mycobacterium marinum during infection reveals genetic requirement for virulence and survival in amoebae and microglial cells

This study uses Tn-Seq to identify essential genes for Mycobacterium marinum survival in amoebae and microglial cells, revealing metabolic and virulence gene requirements during infection.

Disruption of vacuolin microdomains in the host Dictyostelium discoideum increases resistance to Mycobacterium marinum-induced membrane damage and infection

The paper shows that disrupting vacuolin microdomains in Dictyostelium discoideum reduces susceptibility to Mycobacterium marinum infection by impairing membrane damage and pathogen escape.

A TRAF-like E3 ubiquitin ligase TrafE coordinates endolysosomal damage response and cell-autonomous immunity to Mycobacterium marinum.

Co-ordinated Ras and Rac activity shapes macropinocytic cups and enables phagocytosis of geometrically diverse bacteria

This study shows that RGBARG coordinates Ras and Rac activity to shape macropinocytic cups and enable phagocytosis of geometrically diverse bacteria in Dictyostelium discoideum.

Conidial melanin of the human pathogenic fungus Aspergillus fumigatus disrupts cell autonomous defenses in amoebae

This study shows that DHN-melanin on Aspergillus fumigatus conidia reduces phagocytosis by amoebae and delays phagolysosome maturation, enhancing fungal survival.

Lowe Syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum.

This study identifies F&H peptide-containing proteins that interact with OCRL/Dd5P4 in Dictyostelium discoideum, revealing their role in endocytic membrane cycling and potential involvement in Lowe Syndrome phenotypes.

The developmental cycle of Dictyostelium discoideum ensures curing of a mycobacterial infection at both cell autonomous level and by collaborative exclusion

This study shows that Dictyostelium discoideum can cure mycobacterial infections through cell-autonomous mechanisms and collective exclusion during its developmental cycle.

Dictyostelium discoideum flotillin homologues are essential for phagocytosis and participate in plasma membrane recycling and lysosome biogenesis

This study identifies vacuolins in Dictyostelium discoideum as functional homologues of flotillins, essential for phagocytosis, membrane recycling, and lysosome biogenesis.

Zinc intoxication of Mycobacterium marinum during Dictyostelium discoideum infection is counteracted by induction of the pathogen Zn2+ exporter CtpC

This study shows that M. marinum infection induces Zn2+ accumulation in D. discoideum phagosomes, which is counteracted by bacterial upregulation of the Zn2+ exporter CtpC.

Identification of novel anti-bacterial compounds from a chemically highly diverse pathways-based library using phenotypic screens in amoebae host models

This study screens a diverse chemical library in amoeba models to identify anti-bacterial compounds effective against M. marinum and L. pneumophila, finding 64 active compounds with varying efficacy.

PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection

This paper shows that PIKfyve is essential for delivering V-ATPase and proteases to phagosomes, enabling acidification and killing of bacteria, and protecting against Legionella infection.

Think zinc: Role of zinc poisoning in the intraphagosomal killing of bacteria by the amoeba Dictyostelium

The paper investigates zinc poisoning as a bactericidal strategy in Dictyostelium by studying zinc transporters and their role in phagosome zinc accumulation.

ESCRT and autophagy cooperate to repair ESX-1-dependent damage to the Mycobacterium-containing vacuole

The paper shows that ESCRT and autophagy work together to repair damage to the Mycobacterium-containing vacuole caused by ESX-1, preventing bacterial escape.