R. Shrestha

The potential of undersown species identity vs. diversity to manage disease in crops

This study shows that undersown plant species abundance, not diversity, reduces barley disease through aboveground mechanisms, with specific species improving yield without trade-offs.

Impact of alternative splicing on Arabidopsis proteome

This study provides large-scale proteomic evidence that alternative splicing isoforms are translated in plants, showing their impact on protein abundance and plant growth.

Mutation of a highly conserved isoleucine residue in loop 2 of several ????-coronavirus macrodomains indicates that enhanced ADP-ribose binding is detrimental to infection

This study shows that mutating a conserved isoleucine in the macrodomain of MERS-CoV and SARS-CoV-2 enhances ADP-ribose binding but reduces viral replication and pathogenesis.

Chloroplast Methyltransferase Homolog RMT2 is Involved in Photosystem I Biogenesis

The paper identifies RMT2 as a chloroplast methyltransferase homolog involved in photosystem I biogenesis in Chlamydomonas reinhardtii through a high-throughput O2 sensitivity screen.

SECRET AGENT O-GlcNAcylates hundreds of proteins involved in diverse cellular processes in Arabidopsis

The paper identifies 436 O-GlcNAcylated proteins in Arabidopsis, revealing their involvement in diverse cellular processes and crosstalk with O-fucosylation.

Inactivation of Highly Transmissible Livestock and Avian Viruses Including Influenza A and Newcastle Disease Virus for Molecular Diagnostics

This study shows that PrimeStore(R) molecular transport media inactivates viruses by >99.99% without affecting nucleic acid detection, enabling safer and more efficient diagnostic testing.

Cell of origin epigenetic priming determines susceptibility to Tet2 mutation

The paper shows that the epigenetic state of the cell of origin influences susceptibility to Tet2 mutation, with Sox4 promoting dedifferentiation and increased clonal output in hematopoietic stem cells.

A novel phenylpropanoid methyl esterase enables catabolism of aromatic compounds that inhibit biological nitrification

The paper identifies a novel phenylpropanoid methyl esterase that catabolizes aromatic biological nitrification inhibitors, enabling their use as carbon sources by bacteria.

Sorgoleone degradation by sorghum-associated bacteria; an opportunity for enforcing plant growth promotion

The paper identifies bacterial strains capable of degrading sorgoleone, a plant exudate, and characterizes the genetic basis for this trait, offering potential for engineering beneficial plant-microbe interactions.

Toll-like receptor 7 (TLR7)-mediated antiviral response protects mice from lethal SARS-CoV-2 infection

This study shows that TLR7 signaling protects mice from lethal SARS-CoV-2 infection by enhancing antiviral responses and reducing inflammation.

Nuclear Myosin 1 regulates platelet activation and immune response: A novel therapeutic target for hematologic disorders

This study shows that Nuclear Myosin 1 (NM1) regulates hematopoiesis and immune response, with NM1 deletion causing metabolic shifts and impaired blood cell function, suggesting NM1 as a therapeutic target for hematologic disorders.

Inhibition of the DENV2 and ZIKV RNA polymerases by Galidesivir triphosphate measured using a continuous fluorescence assay

This paper measures the inhibition of DENV2 and ZIKV RNA polymerases by Galidesivir triphosphate using a continuous fluorescence assay, finding it equipotent against both with IC50 values of 42 and 47 M.

Reactive myelopoiesis and FX-expressing monocyte-derived macrophages triggered by chemotherapy promote cancer lung metastasis

This study shows chemotherapy-induced reactive myelopoiesis and FX-expressing macrophages promote cancer lung metastasis through CCL2/CCR2 axis and coagulation-inflammation interplay.

ACSM1 and ACSM3 regulate prostate cancer fatty acid metabolism to promote tumour growth and constrain ferroptosis

This study identifies ACSM1 and ACSM3 as AR-regulated factors that promote prostate cancer growth by enhancing fatty acid metabolism and suppressing ferroptosis.

Origin and dynamics of Mycobacterium tuberculosis subpopulations that predictably generate drug tolerance and resistance

This paper identifies drug-tolerant subpopulations in Mycobacterium tuberculosis using a multi-transwell system and barcoding, showing their role in generating resistance.

Development of amidase-dependent pyrazinoic acid prodrugs with activity against pyrazinamide resistant Mycobacterium tuberculosis

This paper develops amidase-dependent pyrazinoic acid prodrugs that bypass PncA activation, showing efficacy against PZA-resistant Mtb.

Single Particle Tracking With Compressive Sensing Using Progressive Refinement Method On Sparse Recovery (spt-PRIS)

This study introduces spt-PRIS, a new single particle tracking method that improves localization in high-density datasets with overlapping PSFs, showing effectiveness for KRAS4b subpopulation analysis.

Deconvoluting signals downstream of growth and immune receptor kinases by phosphocodes of the BSU1 family phosphatases

This study shows that BSU1 phosphatase uses different phosphorylation sites to transduce signals from brassinosteroid and flagellin receptors in plants.

A novel oral GyrB/ParE dual binding inhibitor effective against multidrug resistant Neisseria gonorrhoeae and other high-threat pathogens

The paper describes a novel oral inhibitor, JSF-2659, effective against multidrug-resistant Neisseria gonorrhoeae and other high-threat pathogens, with low potential for resistance development.

Application of Parallel Reaction Monitoring in 15N labeled Samples for Quantification

This study applies parallel reaction monitoring to 15N labeled samples to improve quantification accuracy in proteomics, particularly for low abundance proteins.

15N metabolic labeling quantification workflow in Arabidopsis using Protein Prospector

The paper introduces a 15N metabolic labeling workflow for protein quantification in Arabidopsis using Protein Prospector, highlighting key features for accurate analysis.

Cells protect chromosome-microtubule attachments, independent of biorientation, using an Astrin-PP1 and CyclinB-CDK1 feedback loop

The paper identifies a feedback loop between Astrin-PP1 and CyclinB-CDK1 that stabilizes mono-oriented chromosome-microtubule attachments independent of biorientation.

Multiomics Characterization of Potential Therapeutic Vulnerabilities in Low-grade Serous Ovarian Carcinoma

This study uses multiomics analysis of LGSOC cell lines to identify therapeutic vulnerabilities and biomarkers for MEK inhibitor efficacy.

Autoantibody landscape of advanced prostate cancer

This study characterizes the autoantibody landscape in metastatic castration-resistant prostate cancer using serum epitope repertoire analysis and identifies novel tumor-specific epitopes.

A smartphone microscope method for simultaneous detection of (oo)cyst of Cryptosporodium and Giardia

This paper develops a smartphone-based microscope method to detect Cryptosporodium and Giardia (oo)cysts in vegetables and water, achieving comparable recovery rates to commercial microscopes.

Single cell transcriptomics identifies a unique adipocyte population that regulates bone marrow environment

The paper identifies a unique adipocyte population in bone marrow, MERAs, that regulate bone and vessel homeostasis through single-cell transcriptomics in mice.

Well-Differentiated Papillary Mesothelioma of the Peritoneum is Genetically Distinct from Malignant Mesothelioma

This study identifies distinct genetic mutations in well-differentiated papillary mesothelioma (WDPM) that differentiate it from malignant mesothelioma.

BAP1 Haploinsufficiency Predicts a Distinct Immunogenic Class of Malignant Peritoneal Mesothelioma

The paper identifies BAP1 loss as a marker for a distinct immunogenic PeM subtype, suggesting potential therapeutic targets like immune checkpoint inhibitors.

The requirement for the amino acid co-germinant during C. difficile spore germination is influenced by mutations in yabG and cspA

This study identifies mutations in yabG and cspA that alter the requirement for amino acid co-germinants during C. difficile spore germination, revealing their role in germinant receptor function.

Comprehensive single cell RNAseq analysis of the kidney reveals novel cell types and unexpected cell plasticity

The paper uses single-cell RNA sequencing to identify novel kidney cell types and Notch-mediated plasticity in collecting duct cells, which is defective in chronic kidney disease.