Ou Wang

Quantum algorithm for identifying RNA 3D motifs by processing RNA secondary structure graphs

This paper introduces a quantum algorithm for identifying RNA 3D motifs by processing secondary structure graphs, demonstrating its ability to detect conserved motifs in RNA sequences.

A single-molecule nanopore sequencing platform

This paper introduces an improved nanopore sequencing platform called CycloneSEQ, demonstrating long-duration sequencing and high yield with applications in genomics.

Assembly and analysis of the genome of Notholithocarpus densiflorus

The paper assembles the genome of Notholithocarpus densiflorus and identifies genetic variation across 11 individuals with varying susceptibility to sudden oak death.

MetaTrass: High-quality metagenome assembly on the human gut microbiome by co-barcoding sequencing reads

MetaTrass improves metagenome assembly by binning first and assembling later, producing more high-quality draft genomes from human gut microbiome samples.

SLR-superscaffolder: a de novo scaffolding tool for synthetic long reads using a top-to-bottom scheme

The paper introduces a de novo scaffolding tool for synthetic long reads that improves assembly quality significantly, especially for assemblies from next-generation sequencing reads.

stLFRsv: a germline SV analysis pipeline using co-barcoded reads

stLFRsv detects structural variations using co-barcoded long reads, achieving 74.2% precision and 22.3% recall for deletions in HG002/NA24385.

Robust Benchmark Structural Variant Calls of An Asian Using the State-of-Art Long Fragment Sequencing Technologies

The paper establishes high-confidence structural variant calls in an Asian individual using long-read sequencing technologies and validates them through PCR and Sanger sequencing.

AsmMix: A pipeline for high quality diploid denovo assembly

AsmMix produces high-quality diploid genome assemblies by integrating co-barcoded and single-molecule sequencing data to reduce errors and improve contiguity.

Comparison of long read methods for sequencing and assembly of a plant genome

This study compares three long-read sequencing technologies for de novo assembly of the Macadamia jansenii genome, evaluating contiguity, accuracy, completeness, cost, and DNA requirements.

Haplotype-Resolved Assembly for Synthetic Long Reads Using a Trio-Binning Strategy

The paper introduces HAST, a tool for haplotype-resolved assembly using trio binning, achieving high accuracy and completeness in haplotype reconstruction from synthetic long reads.

A portable and cost-effective microfluidic system for massively parallel single-cell transcriptome profiling

The DNBelab C4 portable, negative-pressure microfluidic device performs high-throughput single-cell transcriptome profiling, resolving species-specific cells and tissue heterogeneity in murine lung and cerebral cortex comparably to existing platforms.

TGS-GapCloser: fast and accurately passing through the Bermuda in large genome using error-prone third-generation long reads

TGS-GapCloser closes gaps in genome assemblies using error-prone long reads, improving contig N50 and gene completeness while being faster than existing tools.

Development of Coupling Controlled Polymerizations by Adapter-ligation in Mate-pair Sequencing for Detection of Various Genomic Variants in One Single Assay

This paper introduces a new mate-pair sequencing method called CP-AL that improves DNA-circularization efficiency and reduces contamination, enabling accurate detection of genomic variants.

Efficient long single molecule sequencing for cost effective and accurate sequencing, haplotyping, and de novo assembly

This paper introduces stLFR, a low-cost method for long DNA sequencing that enables accurate variant calling, haplotyping, and de novo assembly.

3’ Branch Ligation: A Novel Method to Ligate Non-Complementary DNA to Recessed or Internal 3’OH Ends in DNA or RNA

The paper introduces a novel ligation method called 3BL that allows non-complementary DNA to be ligated to 3' ends of DNA or RNA, improving NGS library construction by reducing adapter dimer issues and increasing yield.