Frederick W. Alt

Human antibody heavy chain variable region exon interacts with 3' regulatory region to form a stable somatic hypermutation center

The paper demonstrates that a human antibody heavy chain variable region exon forms a stable somatic hypermutation center (SHM-C) through interaction with 3' regulatory regions, supporting AID access and SHM in non-dividing cells.

B Cell Receptor Signaling Sets Boundaries for Selection of Broadly Neutralizing Antibody Functional Mutations

The paper shows that B cell receptor signaling sets limits on the selection of functional mutations in antibodies, with mid-affinity antigens showing optimal neutralization.

Asymmetric Boost Responses to an HIV-1 Vaccine: Boost-site Controls Secondary B-cell Fates

The paper shows that boosting at the same site as the initial immunization preferentially engages bnAb lineage precursors in secondary germinal centers, while contralateral boosting recruits naive and non-specific memory B cells.

Mouse models with human antibody repertoires for inducing multiple lineages of HIV-1 broadly neutralizing antibodies

This paper develops six mouse models with engineered antibody repertoires to study and optimize strategies for inducing HIV-1 broadly neutralizing antibodies.

Maturation of HIV-1 neutralizing antibodies in a germinal center conditional expression mouse model

This paper develops a mouse model to study and overcome challenges in maturing HIV-1 broadly neutralizing antibodies by expressing a stalled VRC01 intermediate in germinal center B cells.

Ku70 suppresses alternative end-joining in G1-arrested progenitor B cells

The paper shows that Ku70 suppresses alternative end-joining (A-EJ) in G1-arrested progenitor B cells, directing DSB repair toward classical nonhomologous end-joining (C-NHEJ) and preventing translocations.

Induction of Recurrent Break Cluster Genes in Neural Progenitor Cells Differentiated from Embryonic Stem Cells In Culture

The paper investigates RDC-gene breakage in neural progenitor cells derived from embryonic stem cells, finding that many RDCs in primary NSPCs are developmentally influenced and appear in cultured NPCs.