Ming Li

Pseudomonas aeruginosa CRISPR-Cas primes a minimal proline-codon toxin to abort anti-CRISPR phages

The paper shows Pseudomonas aeruginosa uses a CRISPR-Cas system to silence a proline-codon toxin that stops phage growth, revealing a defense mechanism against anti-CRISPR phages.

Nucleoid-associated proteins sense phage-induced genome damage to elicit abortive infection

This study identifies a novel bacterial defense mechanism where nucleoid-associated proteins detect phage-induced genome damage by relocating to the cytoplasm and activating immune effectors.

Construction of a genome-wide pooled CRISPRi library as a resource for exploring the acid tolerance mechanism in Streptococcus mutans

The paper constructs a genome-wide CRISPRi library to identify acid tolerance genes in S. mutans and finds 95 associated genes involved in metabolism, tRNA modification, and transcriptional regulation.

CRISPR-Cas supervises diverse anti-phage defense systems

This study shows CRISPR-Cas regulates diverse anti-phage systems via crlRNAs, balancing their expression to prevent autoimmunity and enhance anti-phage defense.

Phage CRISPR-like regulatory RNAs silence multilayered bacterial immune systems

The paper identifies viral crlRNAs that suppress bacterial immunity by reprogramming Cas proteins and demonstrates their potential for mammalian genome editing.

Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells

This paper develops a compact type I-F2 CRISPR-Cas system for transcriptional activation and base editing in human cells, achieving a wide editing window and high efficiency.