Maria Needhamsen

Advancing Neurological Liquid Biopsy via Six-Base Sequencing of Cerebrospinal Fluid Cell-Free DNA

This paper uses 6-base sequencing to analyze DNA modifications in cerebrospinal fluid cell-free DNA, providing insights into neurodegenerative biology for liquid biopsy applications.

Single-cell analysis suggests coordinated immune-cell redistribution between blood and cerebrospinal fluid in early multiple sclerosis

The paper uses single-cell sequencing to show coordinated immune-cell changes between blood and CSF in early MS, with CSF enrichment of memory T-cells and B-cells and depletion of cytotoxic populations.

Transgenic dCas9-DNMT3A mouse lines enable ex vivo and in vivo methylation editing with locus-dependent transcriptional effects

This study develops CRISPR-based mouse lines for locus-specific DNA methylation editing and shows locus-dependent transcriptional effects in vivo.

EpiATLAS - a reference for human epigenomic research

EpiATLAS provides a comprehensive collection of human reference epigenomes to advance epigenomic research and understanding of cellular function and disease.

Epigenomic profiling of cerebrospinal fluid cells identifies immune regulatory alterations and implicates protocadherins in multiple sclerosis

This study identifies DNA methylation changes in cerebrospinal fluid cells from MS patients, linking them to immune regulatory alterations and implicating protocadherins in MS pathogenesis.

Systematic comparison of dCas9-based DNA methylation epimodifiers over time indicates efficient on-target and widespread off-target effects

This study compares dCas9-based DNA methylation epimodifiers over time, showing efficient on-target effects but widespread off-target methylation and transcriptional changes.

Whole-genome profiling of native 5-hydroxymethylation in human neurons with long-read sequencing

The paper uses long-read sequencing to profile native 5hmC and 5mC in human neurons, showing 5hmC enrichment in transcribed regions and enhancers.

Anoctamin-2-specific T Cells Link Epstein-Barr Virus to Multiple Sclerosis

CD4+ T cells cross-reactive between Epstein-Barr virus EBNA1 and the central nervous system channel anoctamin-2 (ANO2) are elevated in multiple sclerosis and exacerbate experimental autoimmune encephalomyelitis in mice.

Profiling of small non-coding RNAs across cellular and biofluid compartments: implications for multiple sclerosis immunopathology

The paper profiles small non-coding RNAs in multiple compartments of MS patients and controls, identifying distinct sncRNA expression patterns in CSF and PBMCs that suggest organ-specific regulatory mechanisms.