Randall J. Platt

Acquired epithelial Wnt7b secretion establishes autocrine WNT dependency in BRAF mutant colorectal cancer

This study identifies Wnt7b as a critical autocrine WNT ligand required for survival of BRAF-mutant colorectal cancer organoids and shows its association with poor patient survival in human tumors.

Reliable single-cell perturbations explain and improve model performance

The paper evaluates perturbation reliability in single-cell data and shows that using reliable perturbations improves model performance and reduces training data needs.

Cell-stereotyped DNA repair outcomes are widespread during genome editing

The paper develops scOUT-seq to profile DNA repair outcomes in single cells and finds that repair outcomes vary significantly across cell types and subtypes.

Perturbation-aware representation learning for in vivo genetic screens

This paper develops a method to learn perturbation-aware representations from CRISPR screens using single-cell transcriptomics, improving the identification of perturbation effects on gene expression.

An oncoembryology approach uncovers SoxC-driven regulation of colon development and cancer

This study introduces oncoembryology to identify SoxC transcription factors as regulators of colon development and cancer, showing their role in tumor growth and metastasis.

Fast, multiplexable and highly efficient somatic gene deletions in adult mouse skeletal muscle fibers using AAV-CRISPR/Cas9

This study develops a method to efficiently induce somatic gene deletions in adult mouse skeletal muscle fibers using AAV-CRISPR/Cas9, replicating knockout phenotypes without generating knockout mice.

Developmental molecular controls over arealization of descending cortical motor pathways

The paper identifies developmental molecular programs that control the emergence and area-specific distribution of two subtypes of cortical motor projection neurons.

Pooled AAV-CRISPR Screen with Targeted Amplicon Sequencing

The paper introduces PASTAS, a method for in vivo CRISPR screening in mice, and demonstrates its use in identifying cancer driver variants in liver tumors.

Direct in vivo mapping of functional suppressors in glioblastoma genome

This study uses an AAV-mediated CRISPR screen in mice to identify functional suppressors of glioblastoma, revealing novel and canonical tumor suppressors and their interactions.