Helle Bogetofte

STEP-PTMs: Sequential TMT-based Enrichment and Profiling of Post-Translational Modifications

STEP-PTM enables integrated quantitative analysis of proteome, metabolome, and multiple PTM classes from single biological samples using a modular TMT-multiplexed workflow.

Protein-state dysregulation and sex-specific neurodevelopmental signatures in schizophrenia forebrain organoids

This study uses forebrain organoids to show that schizophrenia involves sex-specific protein-state dysregulation, with changes in post-translational modifications and proteomic pathways rather than transcript levels.

Patient-Derived Air-Liquid Interface Forebrain Organoids Reveal Functional Synaptic Deficits in Schizophrenia

This study uses patient-derived organoids to identify synaptic protein and phospho-regulatory deficits in schizophrenia.

Modeling Synaptic Maturation from Growth Cone to Synapse in Human Organoids

This study develops a centrifugation protocol to isolate growth cones and immature synapses from human organoids, capturing synaptic development stages and enabling models for studying synaptic function and dysfunction.

Multi-omic analysis of guided and unguided forebrain organoids reveal differences in cellular composition and metabolic profiles

This study compares guided and unguided forebrain organoids using multi-omic analyses, revealing differences in cellular composition, metabolic profiles, and post-translational modifications.

Temporal proteomic and PTMomic atlas of cerebral organoid development

This study presents a temporal atlas of protein and PTM abundance in cerebral organoids, showing significant changes during development related to neuronal differentiation and energy metabolism.

A novel post-translational proteomics platform identifies neurite outgrowth impairments in Parkinson's disease GBA-N370S dopamine neurons

This study uses a novel proteomics platform to identify PTM dysregulation in GBA-N370S PD neurons, revealing impaired neuritogenesis and a role for tau.

Lipotype acquisition during neural development in vivo is not recapitulated in stem cell-derived neurons

This study shows that mouse brain lipotypes develop in two stages, with cholesterol increasing postnatally, and that stem cell-derived neurons do not fully recapitulate this lipotype.

Identification of bioactive metabolites in human iPSC-derived dopaminergic neurons with PARK2 mutation: altered mitochondrial and energy metabolism.

This study identifies altered mitochondrial and energy metabolism in PARK2 mutant human iPSC-derived dopaminergic neurons through metabolomic analysis.

Lysosomal perturbations in dopaminergic neurons derived from induced pluripotent stem cells with PARK2 mutation

This study investigates lysosomal alterations in PARK2 knockout dopaminergic neurons derived from iPSCs, revealing links between parkin deficiency and lysosomal dysfunction in Parkinson's disease.