Jakob Nilsson

A chemogenetic CRISPR knockout screen identifies a ubiquitin-mediated calcineurin-BMP pathway relay in T cell activation

FAM122A inhibition of PP2A-B55 through a bipartite binding mechanism

This study identifies a bipartite binding mechanism of FAM122A to PP2A-B55, revealing a conserved phosphorylation site involved in cell cycle regulation.

A proteome-wide dependency map of protein interaction motifs

The paper systematically assesses the function of short linear motifs (SLiMs) by mutating and screening them in human cells, identifying essential SLiMs for cell proliferation and uncovering novel classes and binding partners.

POLO kinase inhibits Protein Phosphatase 1 to promote the Spindle Assembly Checkpoint and prevent aneuploidy

This study shows POLO kinase phosphorylates PP187B to inhibit its interaction with MPS1, maintaining SAC activity and preventing aneuploidy during mitosis.

A functional map of phosphoprotein phosphatase regulation identifies an evolutionary conserved reductase for the catalytic metal ions

The paper identifies CYB5R4 as an evolutionarily conserved reductase that activates PP4 and PP6 by reducing their catalytic metal ions, essential for cell viability under oxidative stress.

Substrate recognition principles for the PP2A-B55 protein phosphatase

The paper identifies conserved mechanisms by which PP2A-B55 substrates bind B55 through α-helices and develops a competitive inhibitor to study its function.

Catalytic and non-catalytic functions of DNA polymerase kappa in translesion DNA synthesis

This study identifies catalytic and non-catalytic functions of DNA polymerase kappa in translesion DNA synthesis using CRISPR screening and TLS analysis.

The RZZ complex facilitates Mad1 binding to Bub1 ensuring efficient checkpoint signaling

The paper shows the RZZ complex helps Mad1 bind to Bub1, which is essential for checkpoint signaling, and that this interaction is conserved from yeast to humans.