John R. Engen

Thermodynamic Insights into the WT and Y220C TP53 DBDs Reveals that the Oncogenic Y220C Variant is a Loss of Function Mutation for Zn2+-binding at Physiological Temperature

The paper investigates the thermodynamic effects of the Y220C TP53 mutation on Zn2+-binding and shows that Rezatapopt restores Zn2+-affinity through two pathways.

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry

This study identifies small molecules that stabilize diverse amyloidogenic immunoglobulin light chains by reducing conformational dynamics and proteolysis.

Magnesium as a Conformational Gatekeeper of KRAS: Structural Dynamics and Therapeutic Implications

This study investigates how magnesium regulates KRAS conformational dynamics and nucleotide exchange using HDX-MS and functional assays, revealing Mg2+ as a critical structural stabilizer.

A druggable redox switch on SHP1 controls macrophage inflammation

This paper identifies a druggable redox switch on SHP1 that controls macrophage inflammation by developing a covalent agonist targeting a specific cysteine residue.

How the non-motile kinesin KIF7 adapts conserved kinesin principles for itsfunction in Hedgehog signaling

This study investigates how KIF7, a non-motile kinesin, uses conserved kinesin principles to function as a scaffold in Hedgehog signaling by regulating GLI transcription factors.

An SH3-binding allosteric modulator stabilizes the global conformation of the AML-associated Src-family kinase, Hck

This study identifies PDA1 as an SH3-binding allosteric modulator that stabilizes Hck conformation, potentially enhancing combination therapy with ATP-site inhibitors.