Luke Boulter

DUCK-Net: Automated deep learning segmentation of Ductular Reaction in murine liver injury captures multicellular niche dynamics from H&E morphology

This paper introduces DUCK-Net, a deep learning model for automated segmentation of Ductular Reactions in murine liver injury, showing high accuracy and correlation with spatial transcriptomic data.

JMJD5 regulates metabolism by hydroxylating ISY1, and regulating the Arginine Methyltransferase PRMT6

This paper identifies JMJD5-ISY1-PRMT6 as a pathway regulating metabolism by hydroxylating ISY1 to inhibit PRMT6, showing its role in liver cancer and potential as a therapeutic target.

Epithelial state-transitions permit inflammation-induced tumorigenesis.

This study identifies epithelial state transitions driven by inflammation that promote tumorigenesis, showing that targeting NOTCH signaling can prevent pre-neoplastic to neoplastic transition.

Mitochondrial genomic alterations in cholangiocarcinoma cell lines

This study analyzes mitochondrial DNA mutations in cholangiocarcinoma cell lines and identifies specific SNVs and SVs that may contribute to cancer progression.

Tissue scarring provides a biomechanical framework to promote mammalian bile duct regeneration through the activation of integrin-SRC/FAK signalling.

This study shows that collagen scar formation during bile duct regeneration alters biomechanical properties, activating integrin-SRC/FAK signaling to drive ductular regeneration through a leading-tip of integrin-2-high cells.

Human BioMolecular Atlas Program (HuBMAP): 3D Human Reference Atlas Construction and Usage

This paper describes the construction and usage of the Human Reference Atlas (HRA) v2.0, which integrates multiscale data across 36 organs and provides tools for analysis and visualization.

Planar cell polarity is essential for the architectural patterning of the mammalian biliary tree.

This study shows that planar cell polarity, mediated by VANGL2, is essential for the proper formation and architecture of the mammalian biliary tree.

Fluorescent in vivo editing reporter (FIVER): A novel multispectral reporter of in vivo genome editing

The paper introduces FIVER, a multispectral reporter for in vivo genome editing that detects NHEJ, HDR, and HITI with fluorescent read-outs, demonstrating its utility in primary cells, organoids, and in vivo models.

Non-canonical Wnt signalling initiates scarring in biliary disease

This study identifies non-canonical Wnt signalling as a driver of biliary scarring and shows that inhibiting Wnt ligands reduces fibrosis in cholestatic diseases.