Christopher John Schofield

Structure and dynamics of Burkholderia pseudomallei OXA-57, a distinctive low-efficiency class D β-lactamase with carbapenem-hydrolyzing activity

This paper characterizes OXA-57, a class D β-lactamase from Burkholderia pseudomallei, showing its activity against penicillins and cephalosporins, limited carbapenem hydrolysis, and structural insights into its mechanism.

Nucleolar endonuclease MBLAC2 orchestrates co-transcriptional ribosomal RNA processing and global transcriptional output

This study identifies MBLAC2 as a nucleolar nuclease essential for co-transcriptional rRNA processing and global transcriptional output.

Covalent Inhibition of New Delhi Metallo-β-Lactamases NDM-1 and NDM-5 by 3-Bromopyruvate

This study shows 3-BP covalently inhibits NDM-1 and NDM-5 MBLs, restoring carbapenem activity against resistant bacterial strains.

Drop on fixed target reaction initiation approach for serial and time resolved crystallography

This paper describes a drop on fixed target method for time-resolved serial crystallography, tested with enzymes to improve measurement consistency across facilities.

Acyl-enzyme dynamics, tautomerisation and hydration regulate turnover of carbapenem antibiotics by the OXA-48 β-lactamase

This study investigates how acyl-enzyme tautomerisation, dynamics, and hydration affect carbapenem antibiotic turnover by OXA-48 β-lactamase using X-ray crystallography and molecular dynamics simulations.

Time-resolved ligand dynamics revealed in a β-lactamase using room-temperature serial crystallography

This study uses room-temperature serial crystallography to observe time-dependent ligand dynamics of the β-lactamase CTX-M-15 with avibactam, revealing conformational changes critical for inhibitor reformation.

Inhibition of systemic mammalian metabolism by carnitine mimics from the gut microbiota

This study investigates the systemic effects of microbiome-derived carnitine analogues in mice, showing carnitine depletion and mitochondrial dysfunction due to inhibition of carnitine biosynthesis.

JMJD5 regulates metabolism by hydroxylating ISY1, and regulating the Arginine Methyltransferase PRMT6

This paper identifies JMJD5-ISY1-PRMT6 as a pathway regulating metabolism by hydroxylating ISY1 to inhibit PRMT6, showing its role in liver cancer and potential as a therapeutic target.

Targeting Mycobacterial Transpeptidases: Evaluating the Roles of Ldt and PBP Inhibition in Suppressing Mycobacterium smegmatis

This study evaluates the roles of Ldt and PBP inhibition in suppressing Mycobacterium smegmatis using beta-lactams and LdtMt2 inhibitors, finding stronger growth suppression with Ldt inhibition.

obABPP-HT*: A Precision-Engineered Activity Proteomics Pipeline for the Streamlined Discovery of Deubiquitinase Inhibitors

This paper introduces a high-throughput activity proteomics pipeline for discovering deubiquitinase inhibitors, identifying potent and selective inhibitors with minimal optimization.

Electrostatic interactions define nacubactam potency against OXA-48-like β-lactamases

This paper compares the potency of nacubactam and avibactam against OXA-48-like β-lactamases, finding that nacubactam is less potent due to electrostatic repulsion with Arg214.

Molecular basis for CSB stimulation of the SNM1A DNA repair nuclease

This study identifies how CSB enhances SNM1A nuclease activity during ICL repair by interacting with its nuclease core and altering substrate conformation.

Cardiac stress leads to regulation of Filamin C dimerisation via an ancient phosphorylation-modulated interaction with HSPB7

The paper investigates how cardiac stress regulates Filamin C dimerisation through phosphorylation-modulated interactions with HSPB7, revealing evolutionary conservation of this mechanism.

Studies on the selectivity of the SARS-CoV-2 papain-like protease reveal the importance of the P2' proline of the viral polyprotein

This study investigates how the P2' proline residue affects SARS-CoV-2 PLpro catalytic efficiency using molecular dynamics and substrate analysis.

Discovery of SARS-CoV-2 Mpro Peptide Inhibitors from Modelling Substrate and Ligand Binding

The paper models SARS-CoV-2 Mpro substrate and ligand binding to identify new inhibitor opportunities through computational analysis of binding interactions.

An essential role for dNTP homeostasis following CDK-induced replication stress

The paper shows that disrupting dNTP homeostasis is synthetically lethal with CDK-induced replication stress in fission yeast, leading to genome instability and cell death.