Craig T. Lapsley

De novo assembly of the Trypanosoma congolense genome reveals an organisation influenced by antigenic variation but distinct from Trypanosoma brucei

This paper presents a telomere-telomere genome assembly of Trypanosoma congolense, revealing distinct VSG organization and expression mechanisms compared to Trypanosoma brucei.

Trypanosoma congolense Variant Surface Glycoprotein gene expression occurs in the absence of monoallelic transcription control

This study shows that Trypanosoma congolense expresses multiple Variant Surface Glycoprotein genes in single cells, unlike Trypanosoma brucei, which uses monoallelic transcription.

Restricted expression site use and extreme genome diversification drives trypanosome antigenic variation in chronic bovine infections

The paper analyzes antigenic variation in Trypanosoma brucei during chronic bovine infections, revealing enhanced diversity and a reproducible temporal expression hierarchy of VSGs.

Reduced levels of inositol hexakisphosphate kinase (IP6K) impair life-cycle transitions and the intracellular development of Trypanosoma cruzi within human cardiomyocytes

This study shows that reducing IP6K levels in T. cruzi impairs key life-cycle transitions and intracellular development in human cardiomyocytes, suggesting IP6K as a potential drug target for Chagas disease.

ATR, a DNA damage kinase, modulates DNA replication timing in Leishmania major.

This study shows that ATR modulates DNA replication timing in Leishmania major, promoting genome stability by preventing replication stress-induced chromosome instability.

Nuclear DNA replication in Leishmania major relies on a single constitutive origin per chromosome supplemented by thousands of stochastic initiation events

R-loops acted on by RNase H1 are a determinant of chromosome length-associated DNA replication timing and genome stability in Leishmania

This study shows that R-loops and RNase H1 regulate DNA replication timing and genome stability in Leishmania, linking R-loop homeostasis to chromosome size and replication order.

Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei

This study uses Nanopore sequencing to show that DNA replication compartmentalization in Trypanosoma brucei affects genome stability and instability by influencing VSG gene recombination and chromosome stability.

Genome-wide mapping of RPA1 and RAD9 reveals the management of polycistronic transcription, replication initiation, and responses to replication stress in Leishmania

This study maps RPA1 and RAD9 in Leishmania to understand replication stress response, showing stress hotspots correlate with replication initiation and transcription termination.

Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome

The paper maps R-loop activities across the genome of Trypanosoma brucei, revealing conserved and novel roles in transcription and genome dynamics.

The ATR kinase of Trypanosoma brucei links DNA damage signalling and monoallelic control of surface antigen gene expression during antigenic variation

This study shows that ATR kinase in Trypanosoma brucei is essential for DNA damage signaling and controls monoallelic VSG expression during antigenic variation.