Stefan G. Sarafianos

A novel proviral mechanism for the ISG, Shiftless, during human coronavirus infection

This study reveals that the ISG Shiftless (SHFL) has both antiviral and proviral functions during coronavirus infection, enhancing susceptibility while modulating IFN signaling.

Structural and Mechanistic Basis of F227C-Mediated Hypersusceptibility to Islatravir in HIV-1 Reverse Transcriptase

The paper investigates how the F227C mutation in HIV-1 reverse transcriptase increases susceptibility to islatravir, revealing structural and mechanistic insights into this collateral sensitivity.

Unraveling the Mechanism of HIV-1 Hypersusceptibility to Tenofovir Imparted by Islatravir Resistance Mutations

The paper investigates how HIV-1 resistance mutations enhance susceptibility to tenofovir by analyzing structural and biochemical changes in reverse transcriptase.

Structural Basis of Polypurine Track Strand Displacement by HIV-1 Reverse Transcriptase

This paper reports cryo-EM structures of HIV-1 RT during PPT strand displacement, revealing the mechanism and key residues involved in the process.

HIV-1 Reverse Transcriptase interactions with Long-acting NNRTI, Depulfavirine (VM1500A)

This study reports the crystal structure of HIV-1 reverse transcriptase bound to depulfavirine, showing its extended binding conformation and resistance to common mutations, along with combination efficacy with other antivirals.

Structural, biophysical, and virological mechanistic characterization of HIV-1 capsid-targeting antivirals

The paper characterizes structural, biophysical, and virological properties of HIV-1 capsid-targeting antivirals, showing improved potency and stability through chemical modifications of PF74.

Damaging the conical morphology of HIV-1 capsid by targeting the FG-binding pocket and disfavoring pentameric subunits needed for core closure

This study investigates how ZW-1261, an FGBP-targeting inhibitor, disrupts HIV-1 capsid morphology by affecting pentameric subunits and core closure.

Translocation of HIV capsid core through the Nuclear Pore Complex by affinity gradient

This paper investigates how HIV capsid core translocates through the Nuclear Pore Complex by analyzing FG repeat interactions and identifies an affinity gradient that facilitates unidirectional translocation.

Characterization of antiviral compounds using Bio-Layer Interferometry

This paper optimizes Bio-Layer Interferometry (BLI) protocols to measure binding affinities of antiviral compounds with viral proteins, demonstrating sub-nanomolar KD values and covalent interactions.

Disruption of LEDGF/p75-directed integration derepresses antisensetranscription of the HIV-1 genome

The paper shows that disrupting HIV-1 integrase interactions with LEDGF/p75 increases antisense RNA (asRNA) production in HIV-1 infected cells.

Identification of unique and potent inhibitors of SARS-CoV-2 main protease from DNA-encoded chemical libraries

This paper identifies potent SARS-CoV-2 main protease inhibitors from a DNA-encoded chemical library using affinity-based selection, achieving an IC50 of 30 nM for compound SLL11.