Luiz Pedro S. de Carvalho

Multi-Species, Genome-Wide Metabolic Network Reconstructions Reveal the Basis for Metabolic Versatility in Mycobacteria

The paper develops genome-scale metabolic models for five Mycobacterium species to test if metabolic versatility stems from nutrient uptake capabilities, finding that transporters and substrate availability are key.

Mycobacterium tuberculosis partitions the Krebs cycle under iron starvation

This study shows how Mycobacterium tuberculosis adjusts its Krebs cycle under iron starvation by splitting carbon flux between oxidative and reductive branches and secreting malate.

Time-resolved cryo-EM reveals conformational trajectory of allosteric activation in isocitrate lyase

The paper uses time-resolved cryo-EM to show how acetyl-CoA binding induces conformational changes in ICL2, leading to asymmetric catalytic activity.

Mycobacterium tuberculosis overcomes phosphate starvation by extensively remodelling its lipidome with phosphorus-free lipids

This study shows Mycobacterium tuberculosis remodels its lipidome to survive phosphate starvation by using phosphorus-free lipids and host-derived phosphate sources.

Architecture of an asymmetric mycobacterial short chain/long chain acyl-CoA carboxylase

The paper reveals the asymmetric architecture of a mycobacterial ACCase complex with distinct short-chain and long-chain acyl-CoA carboxyl transferase activities.

Streptococcus pneumoniae accessory capsular genes modulate fitness, pathogenicity and immune evasion

The paper shows that accessory capsular genes in Streptococcus pneumoniae enhance fitness, pathogenicity, and immune evasion without capsule switching.

Antibiotic resistance evasion is explained by rare mutation frequency and not by lack of compensatory mechanisms

The study shows that Mycobacterium tuberculosis does not develop resistance to D-cycloserine due to its ultra-low mutation frequency, not because of a lack of compensatory mechanisms.

Discovery of a novel stereospecific β-hydroxyacyl-CoA lyase/thioesterase shared by three metabolic pathways in Mycobacterium tuberculosis

This study identifies Rv2498c as a multi-functional β-hydroxyacyl-CoA lyase/thioesterase in Mtb involved in three metabolic pathways and shows its deletion attenuates Mtb in a mouse model.